Enhancement of DNA Vaccine-induced Immune Responses by Influenza Virus NP Gene.
- Author:
So Young CHOI
1
;
You Suk SUH
;
Jae Ho CHO
;
Hyun Tak JIN
;
Jun CHANG
;
Young Chul SUNG
Author Information
- Publication Type:Original Article
- Keywords: DNA vaccine; influenza virus NP; adjuvant
- MeSH: Animals; DNA; Immunization; Influenza, Human; Mice; Nucleoproteins; Orthomyxoviridae; T-Lymphocytes; Vaccination
- From:Immune Network 2009;9(5):169-178
- CountryRepublic of Korea
- Language:English
- Abstract: DNA immunization induces B and T cell responses to various pathogens and tumors. However, these responses are known to be relatively weak and often transient. Thus, novel strategies are necessary for enhancing immune responses induced by DNA immunization. Here, we demonstrated that co-immunization of influenza virus nucleoprotein (NP) gene significantly enhances humoral and cell-mediated responses to codelivered antigens in mice. We also found that NP DNA coimmunization augments in vivo proliferation of adoptively transferred antigen-specific CD4 and CD8 T cells, which enhanced protective immunity against tumor challenge. Our results suggest that NP DNA can serve as a novel genetic adjuvant in cocktail DNA vaccination.
