Sequential evolution of IL-17 responses in the early period of allograft rejection.
10.3858/emm.2009.41.10.077
- Author:
Sang Il MIN
1
;
Jongwon HA
;
Chung Gyu PARK
;
Jae Kyung WON
;
Yang Jin PARK
;
Seung Kee MIN
;
Sang Joon KIM
Author Information
1. Department of Surgery, Seoul National University College of Medicine, Seoul 110-799, Korea. sjkimgs@snu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
graft rejection;
interleukin-17;
neutrophils;
T-lymphocytes, regulatory
- MeSH:
Animals;
Antigens, CD/biosynthesis;
Autoimmunity;
Forkhead Transcription Factors/biosynthesis;
Graft Rejection/immunology/*metabolism;
Heart Transplantation;
Interleukin-17/immunology/*secretion;
Mice;
Mice, Inbred BALB C;
Mice, Inbred C57BL;
Neutrophils/immunology/*metabolism/pathology;
T-Lymphocyte Subsets/immunology/*metabolism;
T-Lymphocytes, Regulatory/immunology/*metabolism;
Time Factors;
Transplantation Immunology
- From:Experimental & Molecular Medicine
2009;41(10):707-716
- CountryRepublic of Korea
- Language:English
-
Abstract:
In addition to CD4+CD25+Foxp3+ regulatory T (T(reg)) cells which protect against autoimmune tissue injury, IL-17-producing CD4+ T (Th17) cells have been recently described and shown to play a crucial role in autoimmune injury. It appears that there is a reciprocal developmental pathway between Th17 and T(reg) cells. Although IL-17 is known to be associated with allograft rejection, the cellular source of IL-17 and the nature of Th17 in the context of allograft rejection remain unknown. In the current study, the dynamics of T(reg) and IL-17-producing cells after syngeneic and allogeneic transplantation were examined using a wild-type murine cardiac transplantation model. Ly6G+ cells were found to produce IL-17 during the early postoperative period and CD8+ as well as CD4+ T cells were also found to produce IL-17 during alloimmune response. Graft-infiltrating Ly6G+, CD4+, and even CD8+ cells were found to express IL-17 highly compared to those in spleen. Although the frequencies of Th17 and T(reg) were found to gradually increase in both syngeneic and allogeneic recipients, Th17/T(reg) ratios were significantly higher in recipients with allograft rejection than in syngeneic recipients. In conclusion, IL-17 is produced by neutrophils during the early postoperative period and subsequently by Th17 and CD8+ T cells during allograft rejection. Th17/T(reg) imbalance is associated with the development of allograft rejection. This study would provide basic information on Th17 biology for future investigation in the field of transplantation.
