The adaptor protein LAD/TSAd mediates laminin-dependent T cell migration via association with the 67 kDa laminin binding protein.
10.3858/emm.2009.41.10.079
- Author:
Eunkyung PARK
1
;
Youngbong CHOI
;
Eunseon AHN
;
Inyoung PARK
;
Yungdae YUN
Author Information
1. Department of Life Science, Ewha Womans' University, Seoul 120-750, Korea. YUNYUNG@ewha.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
cell movement;
focal adhesion protein-tyrosine kinases;
integrins;
laminin binding protein 67-kDa, rat;
Sh2d2a protein, mouse
- MeSH:
Adaptor Proteins, Signal Transducing/genetics/*metabolism;
Amino Acids, Diamino/metabolism;
Carrier Proteins/*metabolism;
*Cell Movement;
Humans;
Integrin alpha6/metabolism;
Jurkat Cells;
Mutation;
Protein Binding;
RNA, Small Interfering/genetics;
*Receptor Cross-Talk;
Receptors, Antigen, T-Cell/metabolism;
T-Lymphocytes/*metabolism;
Transgenes;
Two-Hybrid System Techniques
- From:Experimental & Molecular Medicine
2009;41(10):728-736
- CountryRepublic of Korea
- Language:English
-
Abstract:
The adaptor protein, LAD/TSAd, plays essential roles in T cell activation. To further understand the functions of this protein, we performed yeast two-hybrid screening using TSAd as bait and identified 67 kDa laminin binding protein (LBP) as the interacting partner. Subsequently, TSAd-LBP interaction was confirmed in D1.1 T cell line. Upon costimulation by T cell receptor (TCR) plus laminin crosslinking or TCR plus integrin alpha6 crosslinking, LBP was coimmunoprecipitated with TSAd. Moreover, TCR plus laminin costimulation-dependent T cell migration was enhanced in D1.1 T cells overexpressing TSAd but was disrupted in D1.1 cells overexpressing dominant negative form of TSAd or TSAd shRNA. These data show that, upon TCR plus integrin costimulation, TSAd associates with LBP and mediates T lymphocyte migration.
