Interleukin-1beta promotes the expression of monocyte chemoattractant protein-1 in human aorta smooth muscle cells via multiple signaling pathways.
10.3858/emm.2009.41.10.082
- Author:
Jun Hee LIM
1
;
Hee Jung UM
;
Jong Wook PARK
;
In Kyu LEE
;
Taeg Kyu KWON
Author Information
1. Department of Immunology and Chronic Disease Research Center and Institute for Medical Science, School of Medicine, Keimyung University, Taegu 700-712, Korea. kwontk@dsmc.or.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
aorta;
atherosclerosis;
CCL2 protein, human;
interleukin-1beta;
myocytes, smooth muscle;
NF-kappaB;
protein kinase C;
type C phospholipase
- MeSH:
Active Transport, Cell Nucleus/drug effects;
Aorta/pathology;
Atherosclerosis/immunology/metabolism;
Bridged Compounds/pharmacology;
Cell Nucleus/*metabolism;
Cells, Cultured;
Chemokine CCL2/*biosynthesis;
Estrenes/pharmacology;
Genistein/pharmacology;
Humans;
Interleukin-1beta/metabolism;
Myocytes, Smooth Muscle/drug effects/immunology/*metabolism/pathology;
NF-kappa B/*metabolism;
Phospholipases/antagonists & inhibitors;
Protein-Tyrosine Kinases/antagonists & inhibitors;
Pyrrolidinones/pharmacology;
Recombinant Proteins/metabolism;
Signal Transduction/*drug effects;
Thiones/pharmacology
- From:Experimental & Molecular Medicine
2009;41(10):757-764
- CountryRepublic of Korea
- Language:English
-
Abstract:
Monocyte chemoattractant protein-1 (MCP1) plays a key role in monocyte/macrophage infiltration to the sub-endothelial space of the blood vessel wall, which is a critical initial step in atherosclerosis. In this study, we examined the intracellular signaling pathway of IL-1beta-induced MCP1 expression using various chemical inhibitors. The pretreatment of a phosphatidylcholine (PC)-specific PLC (PC-PLC) inhibitor (D609), PKC inhibitors, or an NF-kappaB inhibitor completely suppressed the IL-1beta-induced MCP1 expression through blocking NF-kappaB translocation to the nucleus. Pretreatment with inhibitors of tyrosine kinase or PLD partially suppressed MCP1 expression and failed to block nuclear NF-kappaB translocation. These results suggest that IL-1beta induces MCP1 expression through activation of NF-kappaB via the PC-PLC/PKC signaling pathway.
