Report on the External Quality Assessment Scheme of Hepatitis Viral Markers in Korea, (2016–2017).
10.15263/jlmqa.2018.40.2.51
- Author:
Eun Jung CHO
1
;
Ae Ran CHOI
;
Ji Hyeong RYU
;
So Jeong YUN
;
Woochang LEE
;
Sail CHUN
;
Won Ki MIN
;
Eun Jee OH
Author Information
1. Department of Laboratory Medicine, Uijeongbu St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Uijeongbu, Korea.
- Publication Type:Review
- Keywords:
External quality assessment;
Hepatitis A;
Hepatitis B;
Hepatitis C;
Immunoassay;
Laboratory proficiency testing;
Pooled serum
- MeSH:
Antibodies;
Antigens, Viral;
Biomarkers*;
Hepatitis A;
Hepatitis B;
Hepatitis B Core Antigens;
Hepatitis B Surface Antigens;
Hepatitis B virus;
Hepatitis C;
Hepatitis*;
Immunoassay;
Immunochromatography;
Korea*;
Laboratory Proficiency Testing;
Luminescence
- From:Journal of Laboratory Medicine and Quality Assurance
2018;40(2):51-69
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
As part of the immunoserology program of the Korean Association of External Quality Assessment Service, we organized two trials on the external quality assessment of hepatitis viral markers in 2016 and 2017. The hepatitis viral antigens and antibodies program consisted of 10 test items. We delivered two and three types of pooled sera specimens to 965 and 965 institutions for the first and second trials of external proficiency testing in 2016, respectively. The number of participating laboratories was 915 (94.8%) and 913 (95.0%) in the first and second trials in 2016, respectively. We also delivered three kinds of pooled sera specimens to 936 and 1,015 institutions for the first and second trials of external proficiency testing in 2017, respectively. The number of participating laboratories was 920 (98.3%) and 996 (98.1%) in the first and second trials in 2017, respectively. The most commonly tested items were hepatitis B surface antigen, followed by the antibodies to hepatitis B surface antigen, anti-hepatitis C virus, hepatitis B envelope antigen, antibodies to hepatitis B envelope antigen, anti-hepatitis A virus and antibodies to hepatitis B core antigen. The most frequently used methods for detecting viral markers were the chemiluminescence immunoassay and the electrochemiluminescence immunoassay, but they yielded a few-false positive results due to the matrix effect. The immunochromatographic assay yielded false-negative results for anti-hepatitis A virus due to low sensitivity. Continuous improvement in the quality of viral hepatitis testing through participation in the survey seems necessary.
