TRPV1 Blocking Alleviates Airway Inflammation and Remodeling in a Chronic Asthma Murine Model.
10.4168/aair.2018.10.3.216
- Author:
Joon Young CHOI
1
;
Hwa Young LEE
;
Jung HUR
;
Kyung Hoon KIM
;
Ji Young KANG
;
Chin Kook RHEE
;
Sook Young LEE
Author Information
1. Division of Pulmonology, Department of Internal Medicine, Seoul St. Mary's Hospital, The Catholic University of Korea College of Medicine, Seoul, Korea. sooklee@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
TRPV1 receptor;
capsazepine;
asthma;
airway remodeling
- MeSH:
Airway Remodeling;
Animals;
Asthma*;
Collagen;
Cytokines;
Goblet Cells;
Hyperplasia;
Inflammation*;
Interleukin-33;
Interleukin-5;
Methacholine Chloride;
Mice;
Ovalbumin;
RNA, Small Interfering
- From:Allergy, Asthma & Immunology Research
2018;10(3):216-224
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Asthma is a chronic inflammatory airway disease characterized by airway hyperresponsiveness (AHR), inflammation, and remodeling. There is emerging interest in the involvement of the transient receptor potential vanilloid 1 (TRPV1) channel in the pathophysiology of asthma. This study examined whether TRPV1 antagonism alleviates asthma features in a murine model of chronic asthma. METHODS: BALB/c mice were sensitized to and challenged by ovalbumin to develop chronic asthma. Capsazepine (TRPV1 antagonist) or TRPV1 small interfering RNA (siRNA) was administered in the treatment group to evaluate the effect of TPV1 antagonism on AHR, airway inflammation, and remodeling. RESULTS: The mice displayed increased AHR, airway inflammation, and remodeling. Treatment with capsazepine or TRPV1 siRNA reduced AHR to methacholine and airway inflammation. Type 2 T helper (Th2) cytokines (interleukin [IL]-4, IL-5, and IL-13) were reduced and epithelial cell-derived cytokines (thymic stromal lymphopoietin [TSLP], IL-33, and IL-25), which regulate Th2 cytokine-associated inflammation, were also reduced. Airway remodeling characterized by goblet cell hyperplasia, increased α-smooth muscle action, and collagen deposition was also alleviated by both treatments. CONCLUSIONS: Treatment directed at TRPV1 significantly alleviated AHR, airway inflammation, and remodeling in a chronic asthma murine model. The TRPV1 receptor can be a potential drug target for chronic bronchial asthma.
