Overexpression of miR-155-5p Inhibits the Proliferation and Migration of IL-13-Induced Human Bronchial Smooth Muscle Cells by Suppressing TGF-β-Activated Kinase 1/MAP3K7-Binding Protein 2.
10.4168/aair.2018.10.3.260
- Author:
Yujia SHI
1
;
Xingli FU
;
Qi CAO
;
Zhengdao MAO
;
Yi CHEN
;
Yun SUN
;
Zhiguang LIU
;
Qian ZHANG
Author Information
1. Department of Respiratory Medicine, The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, Changzhou, China. zhangqian_nmu@163.com
- Publication Type:Original Article
- Keywords:
miR-155-5;
asthma;
bronchial smooth muscle cells;
TAB2;
proliferation;
migration
- MeSH:
Asthma;
Blotting, Western;
Cell Proliferation;
Computer Simulation;
Humans*;
In Vitro Techniques;
Interleukin-13;
Interleukins;
Luciferases;
Lung Diseases;
MicroRNAs;
Muscle, Smooth*;
Myocytes, Smooth Muscle*;
Phosphotransferases*;
Real-Time Polymerase Chain Reaction;
Transforming Growth Factors
- From:Allergy, Asthma & Immunology Research
2018;10(3):260-267
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Molecular mechanisms leading to asthma is still ill-defined. Though the function of microRNAs (miRNAs) in asthma was previously reported, the involvement of miR-155 in important features of this disease remains unknown. The present study was designed to uncover the probable involvement of miR-155-5p in the proliferation and migration of IL-13-induced human bronchial smooth muscle cells (BSMCs) and the intrinsic regulatory mechanism. METHODS: The effects of different concentrations of IL-13 on the proliferation and migration of BSMCs as well as the expression of miR-155-5p and its predicted target transforming growth factor (TGF)-β-activated kinase 1/MAP3K7-binding protein 2 (TAB2) were investigated. The effects of miR-155-5p on the proliferation and migration of interleukin (IL)-13-induced BSMCs was determined in vitro using BSMCs transfected with miR-155 mimic/inhibitor and induced by a high concentration of IL-13. The quantitative real-time polymerase chain reaction (qRTPCR) was employed for determining the expression of miR-155-5p and TAB2. Western blotting was applied to analyze the expression of TAB2 at the protein level. Cell proliferation and migration were assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Transwell assays, respectively. RESULTS: The proliferation and migration of BSMCs were dose-dependently increased with IL-13 treatment. Contrariwise, IL-13 dose-dependently inhibited the expression of miR-155-5p in BSMCs. Mechanistic studies showed that inhibition of miR-155-5p further promoted the stimulatory effects of IL-13, whereas overexpression of miR-155 significantly inhibited these effects. In silico studies and luciferase reporter assays indicated that TAB2 was a negatively regulated miR-155-5p target. CONCLUSIONS: These results suggested that miR-155-5p-inhibit the IL-13-induced proliferation and migration of BSMCs by targeting TAB2 and that the IL-13/miR-155/TAB2 pathway could serve as a therapeutic target for pulmonary diseases, especially asthma.
