Cytoprotective Mechanism of Cyanidin and Delphinidin against Oxidative Stress-Induced Tenofibroblast Death.
10.4062/biomolther.2015.169
- Author:
Dae Cheol NAM
1
;
Young Sool HAH
;
Jung Been NAM
;
Ra Jeong KIM
;
Hyung Bin PARK
Author Information
1. Department of Orthopaedic Surgery, School of Medicine and Hospital, Gyeongsang National University, Jinju 52727, Republic of Korea.
- Publication Type:Original Article
- Keywords:
Cyanidin;
Delphinidin;
Apoptosis;
Rotator cuff;
Tenofibroblast
- MeSH:
Anthocyanins;
Apoptosis;
Cell Death;
Rotator Cuff;
Tendons
- From:Biomolecules & Therapeutics
2016;24(4):426-432
- CountryRepublic of Korea
- Language:English
-
Abstract:
Age-related rotator cuff tendon degeneration is related to tenofibroblast apoptosis. Anthocyanins reduce oxidative stress-induced apoptotic cell death in tenofibroblasts. The current study investigated the presence of cell protective effects in cyanidin and delphinidin, the most common aglycon forms of anthocyanins. We determined whether these anthocyanidins have antiapoptotic and antinecrotic effects in tenofibroblasts exposed to H₂O₂, and evaluated their biomolecular mechanisms. Both cyanidin and delphinidin inhibited H₂O₂-induced apoptosis in a dose-dependent manner. However, at concentrations of 100 μg/ml or greater, delphinidin showed cytotoxicity against tenofibroblasts and a decreased antinecrotic effect. Cyanidin and delphinidin both showed inhibitory effects on the H₂O₂-induced increase in intracellular ROS formation and the activation of ERK1/2 and JNK. In conclusion, both cyanidin and delphinidin have cytoprotective effects on cultured tenofibroblasts exposed to H₂O₂. These results suggest that cyanidin and delphinidin are both beneficial for the treatment of oxidative stress-mediated tenofibroblast cell death, but their working concentrations are different.
