Divergent long-terminal-repeat retrotransposon families in the genome of Paragonimus westermani.
10.3347/kjp.2003.41.4.221
- Author:
Young An BAE
;
Yoon KONG
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Paragonimus westermani;
degenerate PCR;
LTR retrotransposon;
mobile activity;
phylogenetic relationship
- MeSH:
Amino Acid Sequence;
Animals;
Cloning, Molecular;
DNA, Helminth/analysis;
*Evolution, Molecular;
*Genome;
Molecular Sequence Data;
Paragonimus/*genetics;
Phylogeny;
RNA-Directed DNA Polymerase/chemistry/genetics;
Retroelements/*genetics;
Sequence Alignment;
Sequence Analysis, DNA;
Terminal Repeat Sequences/*genetics
- From:The Korean Journal of Parasitology
2003;41(4):221-231
- CountryRepublic of Korea
- Language:English
-
Abstract:
To gain information on retrotransposons in the genome of Paragonimus westermani, PCR was carried out with degenerate primers, specific to protease and reverse transcriptase (rt) genes of long-terminal-repeat (LTR) retrotransposons. The PCR products were cloned and sequenced, after which 12 different retrotransposon-related sequences were isolated from the trematode genome. These showed various degrees of identity to the polyprotein of divergent retrotransposon families. A phylogenetic analysis demonstrated that these sequences could be classified into three different families of LTR retrotransposons, namely, Xena, Bel, and Gypsy families. Of these, two mRNA transcripts were detected by reverse transcriptase-PCR, showing that these two elements preserved their mobile activities. The genomic distributions of these two sequences were found to be highly repetitive. These results suggest that there are diverse retrotransposons including the ancient Xena family in the genome of P. westermani, which may have been involved in the evolution of the host genome.
