Evaluation of a Commercial Immuno-Chromatographic Assay Kit for Rapid Detection of IgM Antibodies against Leptospira Antigen in Human Serum.
10.3346/jkms.2018.33.e131
- Author:
Fairuz AMRAN
1
;
Yii Ling LIOW
;
Nurul Atiqah Noor HALIM
Author Information
1. Bacteriology Unit, Infectious Disease Research Centre, Institute for Medical Research, Kuala Lumpur, Malaysia. fairuz@imr.gov.my
- Publication Type:Brief Communication
- Keywords:
Diagnostics;
Leptospirosis;
Immunochromatographic Test;
Serological Tests;
Serum IgM Antibodies
- MeSH:
Agglutination Tests;
Antibodies*;
Diagnosis;
Emergencies;
Humans*;
Immunochromatography;
Immunoglobulin M*;
Korea;
Leptospira*;
Leptospirosis;
Mass Screening;
Sensitivity and Specificity;
Serologic Tests;
Zoonoses
- From:Journal of Korean Medical Science
2018;33(17):e131-
- CountryRepublic of Korea
- Language:English
-
Abstract:
Leptospirosis is a febrile zoonotic disease. Routine diagnosis of leptospirosis is based on the detection of specific antibodies with serological tests. The aim of our study was to determine the usefulness of immunochromatographic assay (ICA), ImmuneMed Leptospira IgM Duo Rapid test kit from Korea, in rapid screening of acute leptospirosis in emergency cases with limited expertise. A total of 197 serum samples (93 positive, 104 negative) were selected randomly. The test has good diagnostic sensitivity 73% and specificity 90%. With positive predictive value of 87% and negative predictive value of 79%, this reassures patients have higher chance of correct diagnosis. This ICA is acceptable for screening of leptospirosis but confirmation with microscopic agglutination test should follow.
