The Detailed Kinetics of Cytomegalovirus-specific T cell Responses after Hematopoietic Stem Cell Transplantation: 1 Year Follow-up Data.
- Author:
Seongman BAE
1
;
Jiwon JUNG
;
Sun Mi KIM
;
Young Ah KANG
;
Young Shin LEE
;
Yong Pil CHONG
;
Heungsup SUNG
;
Sang Oh LEE
;
Sang Ho CHOI
;
Yang Soo KIM
;
Jun Hee WOO
;
Jung Hee LEE
;
Je Hwan LEE
;
Kyoo Hyung LEE
;
Sung Han KIM
Author Information
- Publication Type:Original Article
- Keywords: Cytomegalovirus; ELISPOT; Hematopoietic stem cell transplantation
- MeSH: Cytomegalovirus; Enzyme-Linked Immunospot Assay; Follow-Up Studies*; Ganciclovir; Graft vs Host Disease; Hematopoietic Stem Cell Transplantation*; Hematopoietic Stem Cells*; Kinetics*; Theophylline
- From:Immune Network 2018;18(2):e2-
- CountryRepublic of Korea
- Language:English
- Abstract: The detailed kinetics of the cytomegalovirus (CMV)-specific T cell response in hematopoietic stem cell transplant (HCT) recipients have not yet been fully assessed. We evaluated these kinetics of CMV-specific T cell response and factors associated with high CMV-specific T cell responses 1 year after HCT. In HCT recipients, CMV pp65 and IE1-specific ELISPOT assay were performed before HCT (D0), and at 30 (D30), 90 (D90), 180 (D180), and 360 (D360) days after HCT. Of the 51 HCT recipients with donor-positive (D+)/recipient-positive (R+) serology, 26 (51%) developed CMV infections after HCT. The patterns of post-transplantation reconstitution for CMV-specific T cell response were classified into 4 types: 1) an initial decrease at D30 followed by gradual T cell reconstitution without CMV infection (35%), 2) an initial decrease at D30 followed by gradual T cell reconstitution preceded by CMV infection (35%), 3) failure of gradual or constant T cell reconstitution (26%), and 4) no significant T cell reconstitution (4%). There was no significant difference between ELISPOT counts of D360 and those of D0. High CMV-specific T cell responses at D360 were not associated with high CMV-specific T cell response at D0, CMV infection, ganciclovir therapy, graft versus host disease (GVHD), and immunosuppressant use. In conclusion, there are 4 distinct patterns of reconstitution of the CMV-specific T cell response after HCT. In addition, reconstituted donor-origin CMV-specific T cell responses appeared to be constant until day 360 after HCT, regardless of the level of the pre-transplant CMV-specific T cell response, CMV infection, and immunosuppressant use.
