Mitochondrial 10398A>G NADH-Dehydrogenase Subunit 3 of Complex I Is Frequently Altered in Intra-Axial Brain Tumors in Malaysia.
- Author:
Abdul Aziz MOHAMED YUSOFF
1
;
Fatin Najwa ZULFAKHAR
;
Siti Zulaikha Nashwa MOHD KHAIR
;
Wan Salihah WAN ABDULLAH
;
Jafri Malin ABDULLAH
;
Zamzuri IDRIS
Author Information
- Publication Type:Original Article
- Keywords: Brain tumor; Mitochondrial DNA; ND3 A10398G mutation; Malaysia
- MeSH: Brain Neoplasms*; Brain*; Carcinogenesis; Codon; DNA, Mitochondrial; Electron Transport; Humans; Malaysia*; Mitochondria; Neurodegenerative Diseases; Reactive Oxygen Species; Sequence Analysis, DNA
- From:Brain Tumor Research and Treatment 2018;6(1):31-38
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Mitochondria are major cellular sources of reactive oxygen species (ROS) generation which can induce mitochondrial DNA damage and lead to carcinogenesis. The mitochondrial 10398A>G alteration in NADH-dehydrogenase subunit 3 (ND3) can severely impair complex I, a key component of ROS production in the mitochondrial electron transport chain. Alteration in ND3 10398A>G has been reported to be linked with diverse neurodegenerative disorders and cancers. The aim of this study was to find out the association of mitochondrial ND3 10398A>G alteration in brain tumor of Malaysian patients. METHODS: Brain tumor tissues and corresponding blood specimens were obtained from 45 patients. The ND3 10398A>G alteration at target codon 114 was detected using the PCR-RFLP analysis and later was confirmed by DNA sequencing. RESULTS: Twenty-six (57.8%) patients showed ND3 10398A>G mutation in their tumor specimens, in which 26.9% of these mutations were heterozygous mutations. ND3 10398A>G mutation was not significantly correlated with age, gender, and histological tumor grade, however was found more frequently in intra-axial than in extra-axial tumors (62.5% vs. 46.2%, p < 0.01). CONCLUSION: For the first time, we have been able to describe the occurrence of ND3 10398A>G mutations in a Malaysian brain tumor population. It can be concluded that mitochondrial ND3 10398A>G alteration is frequently present in brain tumors among Malaysian population and it shows an impact on the intra-axial tumors.
