Osteogenic Nanofibrous Coated Titanium Implant Results in Enhanced Osseointegration: In Vivo Preliminary Study in a Rabbit Model.
10.1007/s13770-017-0106-6
- Author:
Siddhartha DAS
1
;
Sandeep GURAV
;
Vivek SONI
;
Arvind INGLE
;
Bhabani S MOHANTY
;
Pradip CHAUDHARI
;
Kiran BENDALE
;
Kanchan DHOLAM
;
Jayesh R BELLARE
Author Information
1. Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai 400076, India.
- Publication Type:Original Article
- Keywords:
Osteogenic nanofibrous coating;
Osseointegration;
Titanium implant
- MeSH:
Animals;
Ascorbic Acid;
Dexamethasone;
Durapatite;
Extracellular Matrix;
Gelatin;
Mesenchymal Stromal Cells;
Microscopy, Electron, Scanning;
Osseointegration*;
Osteoblasts;
Osteogenesis;
Spectrum Analysis;
Stem Cells;
Tibia;
Titanium*;
Wettability
- From:
Tissue Engineering and Regenerative Medicine
2018;15(2):231-247
- CountryRepublic of Korea
- Language:English
-
Abstract:
A titanium implant surface when coated with biodegradable, highly porous, osteogenic nanofibrous coating has shown enhanced intrinsic osteoinductive and osteoconductive properties. This coating mimics extracellular matrix resulting in differentiation of stem cells present in the peri-implant niche to osteoblast and hence results in enhanced osseointegration of the implant. The osteogenic nanofibrous coating (ONFC) consists of poly-caprolactone, gelatin, nano-sized hydroxyapatite, dexamethasone, ascorbic acid and beta-glycerophosphate. ONFC exhibits optimum mechanical properties to support mesenchymal stem cells and steer their osteogenic differentiation. ONFC was subjected to various characterization tests like scanning electron microscopy, Fourier-transform infrared spectroscopy, x-ray diffractometry, thermal degradation, biomineralization, mechanical properties, wettability and proliferation assay. In pre-clinical animal trials, the coated implant showed enhanced new bone formation when placed in the tibia of rabbit. This novel approach toward implant bone integration holds significant promise for its easy and economical coating thus marking the beginning of new era of electrospun osteogenic nanofibrous coated bone implants.
