Clinicopathologic and Prognostic Significance of the Zinc Finger of the Cerebellum Family in Invasive Breast Cancer.
- Author:
Wei HAN
1
;
Cong ZHANG
;
Xiao Jiao GAO
;
Hua Bing WANG
;
Fang CHEN
;
Fang CAO
;
Yong Wei HU
;
Jun MA
;
Xing GU
;
Hou Zhong DING
Author Information
- Publication Type:Original Article
- Keywords: Breast neoplasms; Pathology; Prognosis; ZIC family
- MeSH: Blotting, Western; Breast Neoplasms*; Breast*; Cerebellum*; Disease-Free Survival; Female; Humans; Immunohistochemistry; Mastectomy, Radical; Multivariate Analysis; Pathology; Polymerase Chain Reaction; Prognosis; Zinc Fingers*; Zinc*
- From:Journal of Breast Cancer 2018;21(1):51-61
- CountryRepublic of Korea
- Language:English
- Abstract: PURPOSE: Five members of the zinc finger of the cerebellum (ZIC) family—ZIC1, ZIC2, ZIC3, ZIC4, and ZIC5—have been shown to be involved in various carcinomas. Here, we aimed to explore the clinicopathologic and prognostic roles of ZIC family members in invasive breast cancer patients using immunohistochemical analysis, western blotting analysis, and real-time quantitative polymerase chain reaction (RT-qPCR). METHODS: A total of 241 female invasive breast cancer patients who underwent radical mastectomy between 2009 and 2011 were enrolled. ZIC proteins in 241 pairs of breast tumors and corresponding normal tissues were investigated using immunohistochemistry and the clinicopathologic roles of proteins were analyzed using Pearson's chi-square test. Kaplan-Meier curves and Cox regression analysis were also used to analyze the prognostic value of the ZIC proteins. In addition, 12 pairs of fresh-frozen breast tumors and matched normal tissues were used in the western blotting analysis and RT-qPCR. RESULTS: Only ZIC1 expression in normal tissues was obviously higher than that in tumors (p < 0.001). On multivariate analysis, ZIC1 expression (in overall survival analysis: hazard ratio [HR], 0.405, 95% confidence interval [CI], 0.233–0.702, p=0.001; in disease-free survival analysis: HR, 0.395, 95% CI, 0.234–0.669, p=0.001) was identified as a prognostic indicator of invasive breast cancer. CONCLUSION: ZIC1, but not the other proteins, was obviously decreased in breast tumors and associated with clinicopathologic factors. Thus, ZIC1 might be a novel indicator to predict the overall and disease-free survival of invasive breast cancer patients.
