Comparison of Four Human Papillomavirus Genotyping Methods: Next-generation Sequencing, INNO-LiPA, Electrochemical DNA Chip, and Nested-PCR.
10.3343/alm.2018.38.2.139
- Author:
Pornjarim NILYANIMIT
1
;
Jira CHANSAENROJ
;
Witthaya POOMIPAK
;
Kesmanee PRAIANANTATHAVORN
;
Sunchai PAYUNGPORN
;
Yong POOVORAWAN
Author Information
1. Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok. yong.p@chula.ac.th
- Publication Type:Original Article
- Keywords:
Human papillomavirus;
Genotyping;
Next-generation sequencing;
Nested-PCR;
INNO-LiPA;
Electrochemical DNA chip
- MeSH:
DNA*;
Genotype;
Humans*;
Mass Screening;
Methods*;
Oligonucleotide Array Sequence Analysis*;
Uterine Cervical Neoplasms
- From:Annals of Laboratory Medicine
2018;38(2):139-146
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Human papillomavirus (HPV) infection causes cervical cancer, thus necessitating early detection by screening. Rapid and accurate HPV genotyping is crucial both for the assessment of patients with HPV infection and for surveillance studies. METHODS: Fifty-eight cervicovaginal samples were tested for HPV genotypes using four methods in parallel: nested-PCR followed by conventional sequencing, INNO-LiPA, electrochemical DNA chip, and next-generation sequencing (NGS). RESULTS: Seven HPV genotypes (16, 18, 31, 33, 45, 56, and 58) were identified by all four methods. Nineteen HPV genotypes were detected by NGS, but not by nested-PCR, INNO-LiPA, or electrochemical DNA chip. CONCLUSIONS: Although NGS is relatively expensive and complex, it may serve as a sensitive HPV genotyping method. Because of its highly sensitive detection of multiple HPV genotypes, NGS may serve as an alternative for diagnostic HPV genotyping in certain situations.
