Expression of adhesion molecules on CD34+ cells from steady-state bone marrow before and after mobilization and their association with the yield of CD34+ cells.
- Author:
Karin Zattar CECYN
1
;
Eliza Y S KIMURA
;
Dulce Marta S M LIMA
;
Miyoko YAMAMOTO
;
José Orlando BORDIN
;
José Salvador R DE OLIVEIRA
Author Information
- Publication Type:Original Article
- Keywords: Adhesion molecules; Hematopoietic progenitor cells; Mobilization; Stem cell donor; Multiple myeloma; Non-Hodgkin lymphoma
- MeSH: Bone Marrow*; Cell Adhesion Molecules; Chemokines; Endothelial Cells; Hematologic Neoplasms; Hematopoietic Stem Cells; Humans; Integrin alpha4beta1; Leukapheresis; Lymphocyte Function-Associated Antigen-1; Lymphoma, Non-Hodgkin; Metalloproteases; Multiple Myeloma; Peptide Hydrolases; Stem Cells; Stromal Cells; Tissue Donors; Vascular Cell Adhesion Molecule-1
- From:Blood Research 2018;53(1):61-70
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Cell adhesion molecules (CAMs) expressed on hematopoietic progenitor cells (HPCs), endothelial cells, and stromal cells play a pivotal role in the mobilization of CD34+ cells. Herein, we conducted a non-randomized peripheral blood stem cell (PBSC) mobilization study aimed to compare the potential differences in the expressions of several CAMs and chemokines on CD34+ cells obtained from bone marrow aspirate before and after HPC mobilization from patients with hematologic malignancies and healthy donors. METHODS: Three-color cytofluorometric analysis was used to compare the expressions of CAMs and chemokines in the bone marrow before and after mobilization. RESULTS: For all studied groups, CAM expression among those with good and poor yields of CD34+ cells was significantly correlated with VCAM-1 (P=0.007), CD44 (P=0.027), and VLA-4 (P=0.014) expressions. VCAM-1 (P=0.001), FLT-3 (P=0.001), CD44 (P=0.011), VLA-4 (P=0.001), and LFA-1 (P=0.001) expressions were higher before HPC mobilization than after HPC mobilization. By contrast, the expression of CXCR4 significantly varied before and after mobilization only among those with successful PBSC mobilization (P=0.002). CONCLUSION: We attempted to identify particular aspects of CAMs involved in CD34+ cell mobilization, which is a highly complex mechanism that involves adhesion molecules and matrix metalloproteases. The mechanism by which CD34+ cell mobilization is activated through proteolytic enzymes is not fully understood. We believe that CXCR4, VLA-4, CD44, and VCAM-1 are the most important molecules implicated in HPC mobilization, particularly because they show a correlation with the yield of CD34+ cells collected via large volume leukapheresis.
