Analysis of ImmunoCAP(R) ISAC Results of Chronic Spontaneous Urticaria Patients: Comparison with Skin Prick and the MAST-EIA Tests.
- Author:
Ji Hoon KIM
1
;
Joo Young ROH
;
Jong Rok LEE
Author Information
1. Department of Dermatology, Gachon University School of Medicine, Incheon, Korea. james1024@gilhospital.com
- Publication Type:In Vitro ; Original Article
- Keywords:
Chronic spontaneous urticaria;
ImmunoCAP(R) ISAC;
MAST-EIA
- MeSH:
Allergens;
Causality;
Dermatophagoides farinae;
Dermatophagoides pteronyssinus;
Diagnostic Tests, Routine;
Humans;
Immunoenzyme Techniques;
Sensitivity and Specificity;
Skin*;
Urticaria*
- From:Korean Journal of Dermatology
2015;53(9):684-692
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: In vivo and/or in vitro diagnostic tests to identify possible allergens have been conducted for patients with chronic spontaneous urticaria (CSU) in order to identify any predisposing factors and avoid unnecessary restrictions. OBJECTIVE: The purpose of this study was to evaluate the diagnostic effectiveness of ImmunoCAP(R) ISAC (ISAC) in CSU patients. METHODS: A total of 31 patients with CSU were tested by ISAC and Multiple Allergen Simultaneous Test enzyme immunoassays (MAST-EIA). A skin prick test (SPT) was performed for 17 out of 31 patients. The results of ISAC and MAST-EIA were evaluated based on the SPT results. RESULTS: For 17 patients, with SPT used as the reference standard, ISAC compared with MAST-EIA showed lower sensitivity (22.2% vs. 26.5%), higher specificity (100% vs. 95.7%), higher positive predictive value (100% vs. 52.9%), and lower negative predictive value (86.9% vs. 87.7%). The agreement rate was more favorable for ISAC than for MAST-EIA (87.3% vs. 85.1%; kappa=0.324 vs. 0.263). For 31 patients, the agreement rate between ISAC and MAST-EIA was 83.9% for both Dermatophagoides farinae and Dermatophagoides pteronyssinus. Correlation coefficients (Rho) between ISAC and MAST-EIA for each allergen were 0.690 and 0.675, respectively. CONCLUSION: For CSU patients, specificity, positive predictive value, and the agreement rate of ISAC were similar or superior to those of MAST-EIA. In addition, ISAC provides valuable information on the possible sensitization to different allergen sources by elucidating cross-reactive components. With careful clinical correlations in interpretation, ISAC can be a useful diagnostic tool to detect allergens in CSU patients.
