Spermidine Protects against Oxidative Stress in Inflammation Models Using Macrophages and Zebrafish.
10.4062/biomolther.2016.272
- Author:
Jin Woo JEONG
1
;
Hee Jae CHA
;
Min Ho HAN
;
Su Jung HWANG
;
Dae Sung LEE
;
Jong Su YOO
;
Il Whan CHOI
;
Suhkmann KIM
;
Heui Soo KIM
;
Gi Young KIM
;
Su Hyun HONG
;
Cheol PARK
;
Hyo Jong LEE
;
Yung Hyun CHOI
Author Information
1. Anti-Aging Research Center and Department of Biochemistry, Dongeui University College of Korean Medicine, Busan 47227, Republic of Korea. choiyh@deu.ac.kr
- Publication Type:Original Article
- Keywords:
Spermidine;
Macrophages;
Zebrafish;
Anti-Inflammation;
Anti-Oxidant
- MeSH:
Antioxidants;
Cytokines;
Dinoprostone;
Genes, Regulator;
Inflammation*;
Larva;
Macrophages*;
Necrosis;
Neutrophils;
Nitric Oxide;
Oxidative Stress*;
Reactive Oxygen Species;
Spermidine*;
Zebrafish*
- From:Biomolecules & Therapeutics
2018;26(2):146-156
- CountryRepublic of Korea
- Language:English
-
Abstract:
Spermidine is a naturally occurring polyamine compound that has recently emerged with anti-aging properties and suppresses inflammation and oxidation. However, its mechanisms of action on anti-inflammatory and antioxidant effects have not been fully elucidated. In this study, the potential of spermidine for reducing pro-inflammatory and oxidative effects in lipopolysaccharide (LPS)-stimulated macrophages and zebrafish was explored. Our data indicate that spermidine significantly inhibited the production of pro-inflammatory mediators such as nitric oxide (NO) and prostaglandin E2 (PGE2), and cytokines including tumor necrosis factor-α and interleukin-1β in RAW 264.7 macrophages without any significant cytotoxicity. The protective effects of spermidine accompanied by a marked suppression in their regulatory gene expression at the transcription levels. Spermidine also attenuated the nuclear translocation of NF-κB p65 subunit and reduced LPS-induced intracellular accumulation of reactive oxygen species (ROS) in RAW 264.7 macrophages. Moreover, spermidine prevented the LPS-induced NO production and ROS accumulation in zebrafish larvae and was found to be associated with a diminished recruitment of neutrophils and macrophages. Although more work is needed to fully understand the critical role of spermidine on the inhibition of inflammation-associated migration of immune cells, our findings clearly demonstrate that spermidine may be a potential therapeutic intervention for the treatment of inflammatory and oxidative disorders.
