Combined Extracts of Artemisia and Green Tea, Mitigated Alcoholic Gastritis Via Enhanced Heat-shock Protein 27.
10.4166/kjg.2018.71.3.132
- Author:
Yong Seok KIM
1
;
Migyeong JEONG
;
Young Min HAN
;
Jong Min PARK
;
Sang Oh KWON
;
Seong Pyo HONG
;
Ki Baik HAHM
Author Information
1. Department of Biochemistry and Molecular Biology, Hanyang University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Ethanol;
Gastric damages;
Artemisia extracts;
Green tea extracts;
HSP27
- MeSH:
Alcoholics*;
Artemisia*;
Chronic Disease;
Cyclooxygenase 2;
Ethanol;
Gastritis*;
Heat-Shock Proteins*;
Helicobacter;
HSP27 Heat-Shock Proteins*;
Humans;
In Situ Nick-End Labeling;
Oxidoreductases;
Phospholipases A2;
Rats, Wistar;
Stomach;
Stomach Diseases;
Tea*;
Ulcer
- From:The Korean Journal of Gastroenterology
2018;71(3):132-142
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/AIMS: Several lines of evidence from epidemiologic and laboratory studies have shown that the consumption of Artemisia or green tea extracts (MPGT) is inversely associated with the risk of alcohol-induced damage and other chronic diseases. Supported by previous studies showing that the combined extract of Artemisia and green tea, MPGT, exerted significantly either antioxidative or anti-inflammatory actions against Helicobacter pylori-associated gastric diseases, it was hypothesized that MPGT can offer protection against alcoholic gastritis. METHODS: Ethanol was administered to induce gastric damage in Wistar rats, which had been pretreated with various doses of MPGT, to measure the rescuing action of a MPGT pretreatment against ethanol-induced gastric damage. In addition, the molecular mechanisms for the preventive effects were examined. RESULTS: The MPGT pretreatment (100, 300, and 500 mg/kg) alleviated the ethanol-induced gastric damage, which was evidenced by the significant decrease in calcium-dependent phospholipase A2, MAPKs, and NF-κB levels compared to ethanol alone. Furthermore, the MPGT pretreatment preserved 15-prostaglandin dehydrogenase, whereas cyclooxygenase-2 was decreased significantly. All of these biochemical changes led to the significant alleviation of alcohol-associated gastric mucosal damage. Ethanol significantly increased the TUNEL positivity in the stomach, but MPGT decreased the apoptotic index significantly, which was associated with significantly lower pathological scores of ethanol-induced mucosal ulcerations. The significant protective changes observed alcoholic gastritis with MPGT were related to the increased expression of cytoprotective genes, such as heat-shock protein (HSP)27, HSP60, and PDGF. CONCLUSIONS: The efficient anti-inflammatory, anti-apoptotic, and regenerative actions of MPGT make it a potential nutrient phytoceutical to rescue the stomach from alcoholic gastritis.
