LPS-stimulated Macrophage Activation Affects Endothelial Dysfunction.
- Author:
Naehwan BAEK
1
;
Sohyun SIM
;
Kyung Sun HEO
Author Information
- Publication Type:Original Article
- Keywords: Atherosclerosis; EC inflammation; Lipopolysaccharide; p90RSK
- MeSH: Animals; Aorta; Atherosclerosis; Blotting, Western; Carotid Arteries; Endothelial Cells; Escherichia coli; Foam Cells; Gastrointestinal Microbiome; Human Umbilical Vein Endothelial Cells; Humans; In Vitro Techniques; Inflammation; Injections, Intraperitoneal; Interleukin-6; Macrophage Activation*; Macrophages*; Macrophages, Peritoneal; Mice; Nitric Oxide Synthase Type III; Phosphorylation; Plaque, Atherosclerotic; RNA, Messenger; Serine; Toll-Like Receptor 4; Vascular Cell Adhesion Molecule-1; Vascular Diseases
- From:Journal of Bacteriology and Virology 2018;48(1):23-30
- CountryRepublic of Korea
- Language:English
- Abstract: Intestinal microbiota is involved in the atherosclerotic process by development of an atheromatous core with foam cells in carotid arteries. It has reported that lipopolysaccharide (LPS) from Escherichia coli localizes in human atherosclerotic plaque and causes inflammation via interaction with toll like receptor 4. However, there is no evidence that whether LPS-activated macrophages regulate endothelial cell (EC) function. We evaluated whether LPS-activated macrophage acts as one of the stimulants activating EC and its underlying signaling pathways. Using Western blotting and quantitative reverse transcription-polymerase chain reaction (qRT-PCR), we confirmed that intraperitoneal injection with LPS increases iNOS protein and inflammatory cytokine, TNF-α and IL-6 mRNA expressions. To determine whether LPS-mediated macrophage inflammatory condition affects EC activation and inflammation, human umbilical vein endothelial cells (HUVECs) were incubated with isolated peritoneal macrophages from LPS-injected mice. Interestingly, p90RSK Serine 380 phosphorylation and protein expression were significantly increased by macrophage treatment in EC. Messenger RNA levels of vascular cell adhesion molecule 1 and p90RSK was increased, but endothelial nitric oxide synthase was decreased. In addition, NF-κB promoter activity, which plays an important role in the pathogenesis of inflammation, was strongly enhanced by the macrophage treatment in EC. We further evaluated the effects of LPS on EC function in the mouse aorta using en face staining. In agreement with in vitro result, p90RSK expression was strongly increased in the steady laminar flow region of the mouse aorta in mice injected with LPS. Together, our study demonstrates that p90RSK might be a one of the major therapeutic candidates for the prevention of vascular diseases mediated by LPS.
