Sequencing analysis of HPV-other type on an HPV DNA chip.
10.5468/ogs.2018.61.2.235
- Author:
Min Jeong KIM
1
;
Jin Ju KIM
;
Sunmie KIM
Author Information
1. Department of Obstetrics and Gynecology, Healthcare Research Institute, Gangnam Healthcare Center, Seoul National University Hospital, Seoul, Korea. sunmie@snuh.org
- Publication Type:Original Article
- Keywords:
Papillomaviridae;
Genotype;
Oligonucleotide array sequence analysis;
DNA sequence analysis
- MeSH:
Delivery of Health Care;
DNA*;
Female;
Genotype;
Gynecology;
Human papillomavirus 18;
Humans;
Oligonucleotide Array Sequence Analysis*;
Papanicolaou Test;
Papillomaviridae;
Prospective Studies;
Sequence Analysis, DNA
- From:Obstetrics & Gynecology Science
2018;61(2):235-241
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVES: To identify the specific human papillomavirus (HPV) genotypes from HPV-other type on an HPV DNA chip test by sequencing. METHODS: Among 13,600 women undergoing a routine gynecology examination including Pap smear and/or HPV test by DNA chip test in the healthcare system at Gangnam Center from July 2012 to February 2013, we prospectively collected and performed sequencing for a total of 351 consecutive cervicovaginal samples consisting of 180 samples that tested positive for HPV-other type and 171 samples that tested positive for either high-risk HPV or low-risk HPV. RESULTS: Of a total of 351 samples, individual HPV genotypes were successfully sequenced in 215 cases: 119 HPV-other type, 82 HPV-high-risk, and 14 HPV-low-risk. Based on the sequencing for 119 HPV-other type samples, 91.6% were detected as HPV types that were not included on the DNA chip; however, 7.6% (9/119) were proven to be high-risk HPV types: HPV 18 (n=4), HPV 33 (n=3), HPV 35 (n=1), and HPV 59 (n=1). For correlation analysis of all high-risk and HPV 16/18, the correlation rate was 76.2% and 86.6% with kappa-value of 0.38 and 0.69, respectively. CONCLUSION: HPV-other type on DNA chip test may still have possibility of high-risk HPV, i.e., HPV 18 and thus the significance of HPV-other type in detecting cervical disease remains to be investigated.
