In Vitro Release of Vancomycin from Vancomycin-loaded Demineralized Bone Coated with Hematoma.
- Author:
Kee Hyung RHYU
1
;
Soo Taek LIM
;
Hee Joong KIM
Author Information
1. Department of Orthopedic Surgery, Seoul National University College of Medicine, Seoul, Korea. oskim@snu.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Cancellous Bone;
Demineralized;
Hematoma Coating
- MeSH:
Anti-Bacterial Agents;
Bone Matrix;
Hematoma*;
Osteomyelitis;
Vancomycin*
- From:Journal of Korean Orthopaedic Research Society
2002;5(1):55-60
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: This study was performed to find a method to utilize demineralized bone matrix as a vehicle for local delivery of the antibiotics for the treatment of chronic osteomyelitis. MATERIALS AND METHODS: Bovine bone blocks with the same size were randomly divided into 4 groups. Using Demineralization and hematoma coating, the different characteristics were given to each group, and Vancomycin was adsorbed to these bone blocks. We performed two identical experiments with Phosphate-buffered Saline and Fetal Bovine Serum as a solute. We exchange the solution with 24-hour interval for 6 weeks. The eluted concentrations of Vancomycin at each week were measured. RESULTS: Regardless of the solution used, the eluted concentration of Vancomycin was significantly higher in demineralized and hematoma-coated group than those of other groups. The eluted concentration of Vancomycin were 22.6ng/ml at week 6 in saline condition and 12.4ng/ml at week 5 in serum condition. CONCLUSIONS: We thought that demineralized and hematoma-coated cancellous bone can be used as a good vehicle for local antibiotics delivery.
