MiR-192/-215 Regulates the Biological Functions of Gastric Cancer Cells by Targeting BIVM
- VernacularTitle:MiR-192/-215靶向BIVM基因调控胃癌细胞生物学功能
- Author:
Hui-Juan LIN
1
;
Jiang-Yu ZHANG
;
Meng-Ting YANG
;
Wen-Jing CHEN
;
Hui-Bin LI
;
Jian WANG
;
Xiao-Jing ZHANG
Author Information
1. 广州医科大学附属广东省妇儿医院病理科
- Keywords:
gastric cancer;
miR-192/-215;
BIVM
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2018;39(3):349-355
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To investigate the molecular mechanism of miR-192/-215 targeting BIVM in human gastric cancer.[Methods] First,BIVM was the target gene of miR-192/-215 screened by the target gene prediction in solico and gene microarrays.Real-time quantitative PCR verified the results of the microarrays.Then the double-luciferase reporter plasmids were constructed to test that BIVM was the target gene of miR-192/-215.Subsequently,BIVM-siRNA was transfected,to know the effects of BIVM-siRNA on proliferation and apoptosis of gastric cancer cells.8 nude mice were randomly divided into two groups:BIVM-siRNA experimental group and NSC-siRNA control group.Gastric cancer cells transfected with BIVM-SiRNA were implanted under the skin of nude mice to observe the effect of BIVM on the tumorigenicity of gastric cancer cells.[Results] BIVM was screened as miR-192/-215 target gene by gene microarrays and quantitative PCR.Double-luciferase reporting assays were performed to identify the BIVM as miR-192/-215 target gene.The cell proliferation assays showed that BIVM promoted the proliferation of gastric cancer cells (P＜0.05).Test of flow cytometry showed that BIVM inhibited the apoptosis of gastric cancer cells (P＜0.05).Mouse tumorigenesis test confirmed that BIVM could promote gastric cancer cells growth in vivo (P＜0.05).[Conclusion] BIVM plays a role in the carcinogenesis of gastric cancer,and miR-192/-215 targeting BIVM promotes the development of gastric cancer.
