Expression of MicroRNA-200c in Colorectal Carcinomas and Its Role on Tumor Cell Migration and Invasion
- VernacularTitle:MicroRNA-200c在结直肠癌中的表达及对侵袭和转移的影响
- Author:
Wei-Biao YE
1
;
Yong-Qiang XU
;
Yu-Ling LI
;
Bi-Yan LU
;
Xiang-Ling YANG
;
Huan-Liang LIU
;
Zhong-Jun LI
Author Information
1. 南方医科大学附属东莞人民医院
- Keywords:
microRNA-200c (miR-200c);
colorectal carcinomas;
tumor metastasis;
epithelial-mesenchymal transition
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2018;39(3):335-340
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To investigate the expression of microRNA-200c (miR-200c) in colorectal carcinomas (CRC),and analyze its role on tumor cell migration and invasion.[Methods] The expression levels of miR-200c in CRC tissues and adjacent normal mucosa were assessed by real-time quantitative RT-PCR (qRT-PCR).miR-200c mimics were transiently transfected into human colorectal cancer cells,and their roles on cell migration and invasion were analyzed by Transwell assay.Cell proliferation was measured using the Cell Counting kit-8.The expression levels of epithelial and mesenchymal markers as well as related transcription factor ZEB1 were detected by Western blotting.[Results] Lower miR-200c expression was found in primary CRC tissues with lymph node metastasis compared to those without lymph node metastasis and adjacent normal mucosa.Transfection of miR-200c mimics suppressed proliferation,and reduced invasion and migration in SW620 cells.Furthermore,up-regulation of miR-200c inhibited ZEB1,and resulted in increased E-cadherin and reduced Vimentin gene expression.[Conclusion] miR-200c was associated with invasive and metastatic behavior of CRC.These effects may be mediated through regulation of epithelial-mesenchymal transition.
