Detection of Immune Cell Subsets of Peripheral Blood in Hepatocellular Carcinoma before Liver Transplantation
- VernacularTitle:肝癌患者肝移植前外周血免疫表型分析
- Author:
Xiao-Hong DENG
1
;
Qian-Qian ZHANG
;
Yan CAI
;
Juan OUYANG
;
Mao-Gen CHEN
;
Xiu-Xin TANG
;
Xiao-Shun HE
;
Jun-Qi HUANG
Author Information
1. 中山大学附属第一医院器官移植科
- Keywords:
liver cancer;
liver transplantation;
immunophenotyping;
multi-color flow cytometry
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2018;39(2):178-185,226
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To investigate the characteristics of immunophenotypes in hepatocellular carcinoma(HCC) before liver transplantation.[Methods]The immunophenotypes of T-,B- cells,monocytes,dendritic cells(DC)and NK-cells in peripheral blood from 6 HCC patient who were ready to have liver transplantation and 6 healthy volunteers were analyzed by multicolor flow cytometry.[Results]In the patients,the proportions of CD4+PD-1+T cells,Treg cell (CD4+CD25+CD39+T cells),CD19+B cells,Plasmablasts(CD27highCD38highIgD-IgM-),classical monocytes(CD14high CD16-)and mature NK-cells(CD3-CD56high)were all higher than those in the healthy controls(all P<0.05).However, marginal zone B cell(CD27+IgD+),Non-switched B cells(CD27+CD38dimIgM+),intermediate monocytes(CD14high CD16+)and immature NK-cells(CD3-CD56+)were lower than those in the healthy controls(all P<0.05). And there wasn't any obvious difference in quantity being observed among other cell types.[Conclusion]There was difference in the immunophenotypes of immune cells in peripheral blood between HCC patients before liver transplantation and healthy people.And this finding exerts important effects on monitoring the immune status of the patients after liver transplantation and guiding the administrations of immunosuppressors.
