Regulation of miR-133b on Methamphetamine-induced Neuronal Apoptosis in PC12 Cells
- VernacularTitle:miR-133b在甲基苯丙胺诱导PC12细胞凋亡中的调控作用
- Author:
Hai-Li LIU
1
;
Tao LI
;
Hong-Jie WANG
;
Tao HU
;
Yan-Lai HU
;
Jing ZHANG
;
Jin-Hao SUN
;
Xiao-Guang DONG
Author Information
1. 河北省承德市木兰围场县医院麻醉科
- Keywords:
miR-133b;
methamphetamine;
neuronal injury;
apoptosis
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2018;39(1):26-33
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To investigate the expression changes of miR-133b in methamphetamine(MA)-induced neuro-nal injury in PC12 cells and its regulative effects on cellular apoptosis.[Methods]PC12 cells were cultured and divided into control group and MA treated group.In MA treated group,PC12 cells were insulted with 800μmol/L MA in culture medium. The cellular injury of PC12 cells was observed under microscope. The cellular apoptosis was detected by Hoechst33342/PI double staining,and the expression level of miR-133b was examined by real-time quantitative PCR(RT-PCR). Further-more,miR-133b mimic and inhibitors were transfected into PC12 cells to analyze miR-133b's function in MA-induced cell apoptosis.[Results]The data showed that 800 μmol/L MA could induce obvious cellular injury,cause neurite shortened and increase the cell apoptosis. The RT-PCR data showed that the expression of miR-133b of PC12 cells treated with MA de-creased significantly.The apoptosis rate of PC12 cells decreased after transfection of miR-133b mimic,while increased after transfection of miR-133b inhibitors.[Conclusions]High concentration of MA causes neuron damage and induces neuronal apoptosis,and also decreases the levels of miR-133b expression. Whereas,overexpression of miR-133b can reduce the apoptosis of cultured PC12 cells.Thus,miR-133b plays a crucial role in MA mediated neurotoxicity.This study provides a theoretical basis for elucidating the mechanism of MA-induced neurotoxicity and may provide a new strategy for treating MA addiction.
