Analysis of mitochondrial DNA mutation in leukemia
10.3760/cma.j.issn.1006-9801.2018.07.010
- VernacularTitle:白血病线粒体DNA突变情况分析
- Author:
Jing WANG
1
;
Yongan ZHOU
;
Liping SU
;
Fengmin LI
;
Ming CHEN
Author Information
1. 066000,秦皇岛市第一医院血液科
- Keywords:
Leukemia;
Mitochondrial DNA mutation
- From:
Cancer Research and Clinic
2018;30(7):473-476
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mitochondrial DNA mutation in leukemia.Methods Mitochondrial DNA of 16 leukemia patients in First Hospital of Qinhuangdao from February to June 2017 were amplified and sequenced by using polymerase chain reaction (PCR).The result was compared with revised Cambridge reference sequence (rCRS) and human mitochondrial genome database (mtDB),and the mutation was also analyzed.Results There were 106 mutation genes in total,including 47.17 ％ (50/106) in D-loop region,2.83 ％ (3/106) in ND4 region,17.92 ％ (19/106) in ND5 region,22.64 ％ (24/106) in Cytb region,7.55 ％ (8/106) in ND1 region,1.89 ％ (2/106) in Co Ⅱ region.Conclusion There is a high mitochondrial DNA mutation rate in leukemia patients.
