Study on expression profile of long non-coding RNA in colorectal carcinoma

VernacularTitle:结肠癌中长链非编码RNA表达谱分析
Author: Baocheng ZHAO ¹ ; Zhenjun WANG ; Guanghui WEI ; Jiagang HAN ; Bingqiang YI ; Huachong MA ; Yong YANG
Author Information

1. 100020,首都医科大学附属北京朝阳医院普外科
Keywords: Colonic neoplasms; Long non-cording RNA; Gene expression profiling
From: Cancer Research and Clinic 2018;30(4):217-221
CountryChina
Language:Chinese
Abstract: Objective To analyze the differentially expressed long non-cording RNA (lncRNA) in human colon carcinoma tissue and paracancerous normal colon tissue. Methods Total RNA from the colon carcinoma tissues and paracancerous normal colon tissues of 6 patients from July to August 2015 in Beijing Chao-Yang Hospital, Capital Medical University were prepared respectively. The mRNA of total RNA was amplified, labeled and purified. Hybridization was performed with the profile chip, and the data were normalized and analyzed. Results Compared with normal tissues, 1 339 lncRNAs expressed differentially (P < 0.05), 53 lncRNAs were significantly differential (fold change > 2) in colon carcinoma tissues, in which 41 lncRNAs were up-regulation and 12 lncRNAs were down-regulation.Three lncRNAs had more than 3 times changes. Conclusion Obvious changes of lncRNA expression profile are observed in the pathogenesis of colon carcinoma,suggesting that lncRNA may be related to the progress of colon carcinoma.