Application of next generation sequencing in identifying therapeutic new targets for advanced gastric cancer
10.3760/cma.j.issn.1006-9801.2018.02.004
- VernacularTitle:新一代测序技术在鉴定晚期胃癌治疗新靶点中的应用
- Author:
Na ZHOU
1
;
Dong LIU
;
Chuantao ZHANG
;
Helei HOU
;
Man JIANG
;
Xiaochun ZHANG
Author Information
1. 266000,青岛大学附属医院肿瘤科
- Keywords:
Stomach neoplasms;
Sequence analysis;
Molecular targeted therapy
- From:
Cancer Research and Clinic
2018;30(2):89-92
- CountryChina
- Language:Chinese
-
Abstract:
Objective Next generation sequencing (NGS) was used for identifying the potential gene mutations in advanced gastric cancer(AGC) tissue in order to instruct the targeted drug use for AGC patients. Methods A total of 31 AGC patients from the Affiliated Hospital of Qingdao University between August 10, 2016 and April 23, 2017 were enrolled, and the tissues of gastric cancer were collected. 7 708 exons of 508 tumor related genes and 78 introns of 19 frequently rearranged genes were assessed for base substitutions, indels, copy number alterations, and gene fusions detection. The average median sequencing depth was 470×. Results Somatic mutations were detected in all the patients, of which 9.7 % (3/31) patients could select targeted drugs approved by National Comprehensive Cancer Network (NCCN) guideline according to the identified genetic alterations (human epidermal growth factor receptor 2 gene amplification / mutation). Other targetable genetic alterations were detected in 13 (41.9 %) AGC patients, including mTOR inhibitors, PARP inhibitors, FGFR inhibitors and other targeted drugs of mutations genes. Conclusion The detection of targeted genes based on NGS might help to identify available targeted drugs and to personalize targeted therapy for AGC patients.
