Protective effects of allicin on oxidative stress injury of melanocytes
10.3760/cma.j.issn.1671-0290.2018.04.019
- VernacularTitle:大蒜素对黑素细胞氧化应激损伤的保护作用
- Author:
Yaping TANG
1
;
Yanan KE
;
Yumei LIU
;
Zhenjie LI
;
Yangqi LI
;
Jianqin WANG
Author Information
1. 510095,广州皮肤病防治所
- Keywords:
Melanocytes;
Allicin;
Hydrogen peroxide;
Protective factors;
Oxidative stress
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2018;24(4):282-285
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect and mechanism of garlicin on oxidative stress injury of human melanocytes.Methods There were blank group,control group,hydrogen peroxide group,garlicin group,experiment 1,2 and 3 groups.No cells in the blank group were only added with complete culture medium.The control group was added with complete medium;0.4 mmol/L hydrogen peroxide complete medium was added to the hydrogen peroxide group.Garlicin group was added with freshly prepared garlicin complete culture medium with concentration of 40 μmol/L;Experiment groups 1,2,and 3 were treated with different concentrations of garlicin (80,40,and 20 μmol/L garlicin complete medium,respectively) to interfere with melanocytes treated with 0.4 mmol/ L hydrogen peroxide during logarithmic growth period.After 24 h of drug intervention,the cell morphology was observed under an inverted microscope.The protective effect of garlicin on melanocytes damaged by oxidative stress was measured by MTT colorimetric method.Results Different concentrations of garlicin had different protective effects on melanocytes induced by hydrogen peroxide.It could be concluded that the activity of melanocytes in hydrogen peroxide group decreased significantly (43.610 ± 3.872)％ (P＜0.05),but there was no statistical difference between the two groups (P=0.345).The activity of melanocytes in experimental group 1 was significantly decreased (58.223 ± 2.806) ％ but higher than that in experimental groups 2 and 3 (P＜0.05).Conclusions Allicin inhibits the production of intracellular ROS in human melanocytes induced by H2 O2,regulates oxidative stress in human melanocytes,and counteracts H2O2-induced apoptosis.Therefore,allicin may be a protective factor in mediating oxidative stress in the body.
