Effects of low concentration 5-fluorouracil and triamcinolone acetonide on ear hypertrophic scar in a rat model
10.3760/cma.j.issn.1671-0290.2018.03.018
- VernacularTitle:低剂量5-氟尿嘧啶与曲安奈德对兔耳增生性瘢痕的作用
- Author:
Jianping LYU
1
;
Wenhua FU
;
Shaohua WANG
;
Xiaoliang HAO
;
Yanhua WANG
Author Information
1. 261041,山东潍坊市人民医院美容整形科
- Keywords:
Hypertrophic scar;
5-fluorouracil;
Triamcinolone acetonide;
Vascular endothelial growth factor;
CD34
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2018;24(3):202-205
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of low concentration 5-fluorouracil combined with triamcinolone acetonide on rabbit ear hypertrophic scar model.Methods White rabbits post-traumatic scars were randomly divided into A,B,C and D groups,each group contained 18 cases.Group A was given triamcinolone acetonide and low concentration 5-fluorouracil,Group B was given low concentration 5-fluorouracil,Group C was given triamcinolone acetonide and Group D was used as the control group.The dynamic changes of the scar were observed by naked eyes,hematoxylin-eosin (HE) staining and van Gieson (VG) staining through microscope.Vascular endothelial growth factor (VEGF) and CD34 were detected and compared within 4 groups by immunohistochemical staining.Results We successfully established a rabbit ear scar model.It was observed that the scars of Groups A,B and C had been shorter and flatter than Group D,close to the normal tissue.In HE and VG staining,the number of fibroblasts,inflammatory cells,blood vessels in groups A,B and C had been decreased,compared with Group D.Immunohistochemical results showed that the positive rates of VEGF and CD34 from the most to the lest were Groups D,B,C and A.The scar proliferation index from the most to the lest were Groups D,B,C and A.Conclusions The effect of low concentration 5-fluorouracil in treatment of the rabbit ear hypertrophic scar is better than that of triamcinolone acetonide.Low concentration 5-fluorouracil combined with triamcinolone acetonide has a synergistic effect in treatment of hypertrophic scar,which has better effect than that of low concentration 5-fluorouracil or triamcinolone acetonide alone.
