Effect of different dosage of recombinant human basic fibroblast growth factor on skin and muscle
10.3760/cma.j.issn.1671-0290.2018.02.018
- VernacularTitle:不同剂量外用重组人碱性成纤维细胞生长因子对皮肤和肌肉的影响
- Author:
Tingting HE
1
;
Chen ZHANG
;
Shibing JING
;
Shenwei LIU
;
Bo HU
Author Information
1. 116000,大连大学整形外科研究所
- Keywords:
Rh-bFGF;
Skin;
Gastrocnemius
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2018;24(2):130-134
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of external recombinant human basic fibroblast growth factor (rh-bFGF) on the skin and muscles.Methods 40 BALB/c mice of 6 weeks were randomly divided into skin and muscle groups.The skin group was randomly divided into group A and group B.The skin group was injected with 100U rh-bFGF and the same dose of saline in the chin.The muscle groups were randomly divided into C,D,E,and F,and the muscle group was injected into the left calf muscle in order of 200,400,800U rh-bFGF and the same dose of normal saline.The materal was taken at week 8.HE staining was used to observe the submental tissue and muscle fiber morphology.Two groups of muscle fibers,collagen and fibrous tissue were detected by trichrome stain.Immunohistochemistry was used to determine the blood vessel density of the chin and gastrocnemius.Results In week 8,10 mice in group A had a mass in the lower jaw,and the gastrocnemius of the groups C and D showed hypertrophy.Group A showed the thickness of epidermis,dermal papillary layer and mucosa,hair follicle and blood vessel quantity,glandular cavity,collagen and fiber content were sigificantly greater than that of group B (P＜0.05).In the groups C,D and E,the gastrocnemius muscle was significantly thicker than that of group F,and the thickening of group D was the most obvious (P＜0.05),and the contents of collagen and fiber in the groups C,D and E were significantly higher than that in group F.Conclusions Subcutaneous injection of rh-bFGF leads to submental skin thickening,vascular hyperplasia,increased diameter of hair follicle and higher collagen and fiber contents;intramuscular injection of it may induce the muscular hypertrophy and vascular proliferation.
