Effect of adipose-derived stem cell conditioned medium on UVB damage of human dermal fibroblasts in different senescent degrees

VernacularTitle:脂肪干细胞条件培养基对不同衰老程度成纤维细胞中波紫外线损伤的修复效应
Author: Ting WANG ¹ ; Shu GUO ; Peng CHEN ; Zheng CAO ; Shuangyi ZHANG ; Xuehui LIU
Author Information

1. 中国医科大学附属第一医院整形外科
Keywords: Adipose-derived stem cells conditioned medium; HDFs; Cellular senescence
From: Chinese Journal of Medical Aesthetics and Cosmetology 2018;24(2):79-83
CountryChina
Language:Chinese
Abstract: Objective To study the differences of UVB damage among yong,intermediate and aged human dermal fibroblasts (HDFs),the recovery effect of adipose-derived stem cell conditioned medium (ADSC-CM) on UVB damage and the relationship between the recovery effect and ADSC-CM concentration as well as its treating period.Methods HDFs were cultured at different senescent degrees in vitro to establish UVB damage model of HDFs,and then the HDFs were cultured in different concentration ADSC-CM and cellular proliferation was detected.Results The optimal treating period and concentration of ADSC-CM were 48h and 100％ respectively.As to HDFs in different senescent degrees,ADSC-CM was able to accelerate cellular proliferation in both UVB group and control group.Conclusions Independently or simultaneously,the intrinsic and extrinsic factors can cause ageing damages on HDFs.To some extent,ADSC-CM has recovery effect on the damages through secretomes from ADSC.