Effect of fractional laser on the expression of SCF and c-kit in skin stem cells of guinea pig
10.3760/cma.j.issn.1671-0290.2017.06.017
- VernacularTitle:点阵激光对豚鼠皮肤干细胞因子及其c-kit受体表达的影响
- Author:
Ming TAN
1
;
Bo DUAN
;
Song YE
;
Dan WU
;
Shijing ZHOUYAN
Author Information
1. 湖北省妇幼保健院医疗美容科
- Keywords:
Fractional laser;
Stem cell factor;
C-kit receptor;
Guinea pig
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2017;23(6):415-418
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of fractional laser on the expression of stem cell factor (SCF) and c-kit receptor in body surface of guinea pig.Methods 20 guinea pigs were adopted.The back skin of each guinea pig was divided into four zones:A zone (the skin was not treated),B zone (the skin was treated by fractional laser at a low fluence:20 mJ/cm2),C zone (the skin was treated at a medium fluence:60 mJ/cm2),and D zone (the skin was treated at a high fluence:120 mJ/cm2).The immunohistochemical method was used to detect the expression of SCF and c-kit in body surface of guinea pig.The results were quantified using an HPIAS-1000 system.Results One week later,the SCF expression level in B,C and D groups was increased obviously,which showed statistical difference compared with A group (P＜0.05);the c-kit expression level in C and D groups was increased obviously,compared with A group (P＜0.05).4 weeks later,the SCF expression level in C and D groups was higher than A and B groups;furthermore the level in D group was higher than C group (P＜0.05);the c-kit expression level in D group was higher than A and B groups (P＜0.05).Conclusions The fractional laser could generate the curative effect by increasing the expression of SCF and c-kit in body surface.
