Peptides releasing study of serum apolipoprotein A-I and apolipoprotein B by ID-LC-MS
10.3760/cma.j.issn.1009-9158.2018.11.016
- VernacularTitle:ID-LC-MS检测血清载脂蛋白A-I和B载脂蛋白的多肽释放研究
- Author:
Qing LI
1
;
Yi JU
;
Hewei SUN
;
Zhonggan JING
Author Information
1. 200126,上海市临床检验中心 参考测量实验室
- Keywords:
Apolipoprotein A-I;
Apolipoproteins B;
Kinetics;
Chromatography,liquid;
Tandem mass spectrometry;
Isotope labeling
- From:
Chinese Journal of Laboratory Medicine
2018;41(11):870-874
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the digestion kinetics of Apolipoprotein A-I and B by ID-LC-MS method for accurate quantification of proteins .Methods Methodological research .The target peptides of ApoA-I and B were determined .The ApoA-I and B from 5 human serum samples on market with levels from 0.90-2.54 g/L and 0.54-1.39 g/L separately , were measured in terms of target peptides by isotope dilution liquid chromatography mass spectrometry method .The releasing amount and rate of peptides were analyzed and plotted according to different time points .The correlation coefficient R2 was calculated among peptide releasing amount between samples .Results Most peptides reached their peaks within 4 hours.The peptides VQ , DY and VS from Apo A-I, TR and FP from Apo B were released relatively slowly .After getting to their peak stage , the ratio between TEV and SIL-TEV, AK and SIL-AK, VQ and SIL-VQ presented stable state.As for Apo A-I the correlations among peptides are high , from 0.904 to 0.999.Some peptides from Apo B show lower correlations , such as TG-SV with R20.543 (3 h).Conclusions Peptides from Apo A-I and Apo B present different releasing properties after trypsin digestion .Proper selection of representative peptides and enzymatic conditions can benefit accurate quantification of target proteins .
