Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry to differentiate Candida
10.3760/cma.j.issn.1009-9158.2018.04.012
- VernacularTitle:应用基质辅助激光解析电离飞行时间质谱鉴定假丝酵母菌
- Author:
Jing YANG
1
;
Yu SHI
;
Shangrong FAN
;
Yuxia ZHU
;
Xiaoping LIU
Author Information
1. 518036,北京大学深圳医院妇产科
- Keywords:
Candidiasis,vulvovaginal;
Candida;
Spectrometry,mass,matrix-assisted laser desorption-ionization
- From:
Chinese Journal of Laboratory Medicine
2018;41(4):296-299
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the efficiency of matrix assisted laser analytical ionization time of flight mass spectrometry(MALDI-TOF-MS)for identification of Candida species which caused vulvovaginal candidiasis(VVC).Methods Candida strains from VVC were first identified by PCR amplification of the ITS regions and transposable intron of DNA and sequencing analysis.MALDI-TOF-MS wasfurther performed to identify the strainsconfirmedby molecular methods, and at the same time the MALDI-TOF-MS identification database of C.albicans complex was set up.Results In total, 324 Candida strains were identifiedby molecular methods from VVC samples,which encompassed20 different yeast species, including C.albicans,C.africana,C.dubliniesis, C.glabrata, C.bracarensis, C.nivariensis, C.guilliermondii, C.lusitaniae,C.krusei,C.kefyr,C.parapsilosis,C.metapsilosis,C.tropicalis,Kodamaea ohmeri,C. fabianii, Rhodotorula mucilaginosa, Saccharomyces cerevisiae, and Trichosporon asahii.314of all 324 Candida strains were correctly identified by MALDI-TOF-MS.3 C.albicans,1 C.africana,1 C.glabrata, 2 C.metapsilosis,1 C.guilliermondii and 1 Saccharomyces cerevisiae were incorrectly identified, while 1 Torulaspora pretoriensiscannot identified.The accuracy ofidentification was 96.91 %(314/324).97.95%(191/195)of Candida strains, including 153(98.08%)C.albicans, 37(97.37%)C.africanaand 1 (100%)C.dubliniesis, can be correctly identifiedby the newbuiltC.albicans complex database. Conclusion MALDI-TOF-MS is proved to be a rapid and reliable method for identification of Candida strains from VVC,which also has advantages of identification of Candida complex.(Chin J Lab Med,2018, 41:296-299)
