The comparison of different immunoassays for the detection on autoantibodies specific to primary biliary cholangitis
10.3760/cma.j.issn.1009-9158.2018.03.006
- VernacularTitle:不同免疫方法检测原发性胆汁性胆管炎特异性自身抗体的对比研究
- Author:
Haiping ZHANG
1
;
Huiping YAN
;
Xiaosan CHEN
;
Le LIU
;
Qingchun LI
;
Yingxue MA
;
Lijuan LI
;
Jiyang LI
;
Jinli LOU
Author Information
1. 100069,首都医科大学附属北京佑安医院临床检验中心
- Keywords:
Chemiluminescent immunoassay;
AMA-M2;
anti-gp210;
anti-sp100;
Line immunoassay;
Enzyme-linked immunosorbent assay;
Primary biliary cholangitis
- From:
Chinese Journal of Laboratory Medicine
2018;41(3):203-207
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the test performance of different immunoassays for the detection on autoantibodies specific to primary biliary cholangitis,including anti-mitochondrial type 2 antibody(AMA-M2),anti-glycoprotein 210(anti-gp210)and anti-nuclear body protein sp100(anti-sp100).Methods Serum samples from Primary Biliary Cholangitis(PBC, n=91), liver disease control(including viral hepatitis,autoimmune hepatitis and liver cirrhosis,n=67)and healthy individual(n=40)were collected from Beijing Youan Hospital during the period between April 2014 and April 2017.All samples were tested with chemiluminescent immunoassay(CLIA)and enzyme linked immunosorbent assay(ELISA)for AMA-M2, meanwhile the detection on anti-gp210 and anti-sp100 were compared between CLIA and Line Immunoassay(LIA).The Kappa coefficient were used to measure the level of qualitative agreement between different assays.The diagnostic accuracy of AMA-M2 detected with CLIA and ELISA were compared by receiver operating characteristic curve(ROC).Results The overall qualitative agreement between CLIA and ELISA for the detection to AMA-M2 is 88.4%(Kappa =0.765, P<0.01).Excellent qualitative agreement between CLIA and LIA for the detection to anti-gp210 and anti-sp100 was also found with overall agreement as 96.5%(Kappa=0.852,P<0.01)and 98%(Kappa=0.884,P<0.01), respectively.The ROC analysis also showed similar area under the curve(AUC)for CLIA(0.965, P<0.01)and ELISA (0.928,P<0.01)on detection to AMA-M2.Conclusions CLIA and ELISA showed excellent agreement for the detection to AMA-M2.High qualitative agreement between CLIA and LIA was also found when testing anti-gp210 and anti-sp100.
