Study on the correlation of CD8 +T cell with CD8low T cell subsets and CD8high T cell subsets
10.3760/cma.j.issn.1009-9158.2018.01.011
- VernacularTitle:临床回报CD8+T细胞结果与CD8 low T和CD8 high T细胞亚群的相关性研究
- Author:
Yali CHEN
1
,
2
;
Yajing FU
;
Tian TANG
;
Wen ZHAO
;
Ya′nan WANG
;
Wenqing GENG
;
Yongjun JIANG
;
Zining ZHANG
Author Information
1. 110001 沈阳,中国医科大学附属第一医院检验科卫生部艾滋病免疫学重点实验室
2. 山东省胸科医院检验科工作
- Keywords:
CD8-positive T-lymphocytcs;
T-lymphocytes subsets;
Flow cytometry;
Autoanalysis
- From:
Chinese Journal of Laboratory Medicine
2018;41(1):47-52
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between the CD 8 +T cells results of clinical automatic analysis platform and the CD8lowT and CD8highT cell subsets.Methods A total of 1316 cases of lymphocyte and flow cytometry data were collected from the First Affiliated Hospital of China Medical University from December 2015 to September 2016 by cross-sectional study. There were 287 cases of malignant tumor , 389 cases of autoimmune disease , 320 healthy people and 320 cases of HIV infection , then to get automatic analysis platform returns result of CD 8+T cell.FlowJo software was used to analyze the CD8low T and CD8high T lymphocyte subsets in the patients , and the results were compared with the results of CD8 +T cells returned by the clinical automatic analysis platform .Results The results of clinical returns CD8 +T cells were consistent with the results of CD 8high T cells in patients with different diseases , and were not exactly the same as the results of CD8lowT cells, and the difference was as follows:the results of CD8low T cells in HIV-infected patients were significantly lower than those of healthy people (56.2 ±42.0, 68.8 ± 45.9, cells/μl P<0.001), which were different from the clinical results of CD 8 +T cells.The results of clinical report of CD8 +T cells were statistically correlated with CD8high T cells and CD8low T cells, and the correlation between CD8 +T cells and CD8highT were higher than that of CD8lowT cells.There was a positive correlation between CD8low T cell count and CD4 +T cell count ( r=0.204, P<0.001) .CD8low T was significantly higher in patients on antiviral treatment than that in untreated group (58.3 ±43.9, 42.9 ± 26.5, cells/μl, P<0.001).After treatment for more than 2 years, the CD8lowT cells in patients with CD4<500 cells/μl were significantly lower than those in patients with CD 4>500 cells/μl (50.1 ±47.0, 66.3 ±46.6, cells/μl, P<0.001).Conclusions The clinical report of CD8 +T cells was consistent with the results of CD8highT cells, and there was a great difference between the results of CD 8lowT cells and the results of CD8 +T cells.CD8low T cells were significantly reduced in HIV infected patients , and CD8low T cells could be effectively reconstructed by antiviral therapy .
