Expression of triggering receptor expressed on myeloid cells in ischemic optic neuropathy
10.3760/cma.j.issn.1005-1015.2018.05.012
- VernacularTitle:髓样细胞表达触发受体在缺血性视神经病变中的表达
- Author:
Fanchao MENG
1
;
Shilong SUN
;
Jian SU
;
Peiyu SHI
;
Weimin ZHOU
;
Shuxiang WANG
;
Jinting CHU
Author Information
1. 450006,郑州市第二人民医院神经内科
- Keywords:
Optic neuropathy,ischemic;
Triggering receptors expressed by myeloid cells;
Caspase-3;
IL-6;
Animal experimentation
- From:
Chinese Journal of Ocular Fundus Diseases
2018;34(5):471-474
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of triggering receptor expressed on myeloid cells (TREM),Caspase-3 and interleukin (IL)-6 in optic nerve tissue of ischemic optic neuropathy (ION).Methods Twenty Sprague-Dawley rats were randomly divided into control group and model group,10 rats in each group.The permanent ligation of bilateral internal carotid arteries (BICA) was performed for 14 days to establish subacute ION model as model group.The control group were separated BICA without ligation.The expressions of TREM-1,TREM-2,Caspase-3 and IL-6 in rat retina were detected by reverse transcription PCR and Western blot,respectively.Results Compared with the control group,the expressions of TREM-1,Caspase-3,IL-6mRNA (t=6.058,7.86,6.055) and protein (t=9.671,9.524,14.501) in the optic nerve tissue of the model group were increased,while the expression ofTREM-2 mRNA and protein (t=9.283) was decreased,and the difference was statistically significant (P＜0.05).Conclusion In ischemic optic nerve tissue,TREM-1 mRNA and protein were significantly expressed,the expressions of TREM-2 mRNA and protein decreased significantly.
