The influence of human umbilical cord mesenchymal stem cells transplanted into the tail vein of diabetic rats on apoptosis of retinal neurons and the retinal expression of glial fibrillary acidic protein
10.3760/cma.j.issn.1005-1015.2018.02.013
- VernacularTitle:尾静脉注射人脐带间充质干细胞对早期糖尿病大鼠视网膜神经细胞凋亡及胶质纤维酸性蛋白表达的影响
- Author:
Tianling SONG
1
;
Xuxia MENG
;
Bowen CAO
;
Qingmin GUO
;
Xianhui ZHOU
;
Die HU
Author Information
1. 266500,青岛大学附属医院眼科
- Keywords:
Diabetic retinopathy;
Mesenchymal stem cells;
Neurons;
Apoptosis
- From:
Chinese Journal of Ocular Fundus Diseases
2018;34(2):164-167
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of human umbilical cord mesenchymal stem cells (hUCMSC) transplanted into the tail vein of diabetic rats on apoptosis of retinal neurons and the retinal expression level of glial fibrillary acidic protein (GFAP).Methods Seventy clean male Sprague-Dawley rats were randomly divided into the normal control group (group A),diabetes mellitus (DM) only group (group B),DM + balanced salt solution (BSS) group (group C),DM + hUCMSC group (group D),with 10 rats in each group.DM rats were induced by intraperitoneal injection of streptozotocin.Apoptosis of retinal cells was assayed by dUTP nick end labeling.Immunohistochemistry and Western blot was performed to detect the retinal expressions of GFAP in rats.Results Compared with group A,large numbers of apoptotic cells could be found in the retinal ganglion cell layer (GCL) and inner nuclear layer (INL) of group B and group C,however the apoptotic cells in group D were significantly reduced than group B and C.The expression of GFAP was mainly located in the retinal GCL and retinal nerve fibre layer (RNFL) in group A,throughout the inner plexiform layer (IPL) in group B and C,only distributed in RNFL and GCL in group D.It was obvious that the expression of GFAP in group B and C was higher than group A.Compared with group B and C,the expression of GFAP in group D was significantly reduced.The difference of GFAP expression among the 4 groups was significant (F=79.635,P＜0.05).Conclusion hUCMSC could inhibit the apoptosis of retinal cells and activation of glial cells in early DM rats.
