Study on expression profile of long non-coding RNA in chronic thromboembolic pulmonary hypertension
10.3760/cma.j.issn.1001-4497.2018.08.011
- VernacularTitle:慢性血栓栓塞性肺动脉高压中长链非编码RNA表达谱的研究
- Author:
Xiaowei HU
1
;
Pixiong SU
;
Yan LIU
;
Xitao ZHANG
;
Jun YAN
;
Xiangguang AN
;
Song GU
Author Information
1. 100020,首都医科大学附属北京朝阳医院心脏中心 高血压病研究北京市重点实验室
- Keywords:
Long non-coding RNA;
Gene microarray;
Chronic thromboembolic pulmonary hypertension
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2018;34(8):487-490
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the aberrant expression of long non-coding RNA(lncRNA) in chronic thromboembolic pulmonary hypertension,and explore the lncRNA role in pathogenesis of CTEPH.Methods A total of 5 pulmonary artery endarterium tissue of CTEPH patients and 5 pulmonary artery endarterium tissue of healthy controls were collected.Using high-throughput gene microarray technology to detect two groups of lncRNA and mRNA expression spectrum,build lncRNA-mRNA express network,Pathway and GO analysis to explore the gene function.Results Differential expression of 185 lncRNA was observed in the CTEPH tissues compared with healthy control tissues.Further analysis identified 464 regulated enhancerlike lncRNA and overlapping,antisense or nearby mRNA pairs.Coexpression networks were subsequently constructed and investigated.The expression levels of the lncRNA,NR_036693,NR_027783,NR_033766 and NR_001284,were significantly altered.Gene ontology and pathway analysis demonstrated the potential role of lncRNA in the regulation of central process,including inflammatory response,response to endogenous stimulus and antigen processing and presentation.Conclusion Differentially expressed lncRNA may exert a partial role in CTEPH,the results of this study will help in the future about diagnosis and treatment of CTEPH.
