The effect of Puerarin on GAP-43 protein and NGF protein expression of spinal motoneurons following brachial roots avulsion
10.3760/cma.j.issn.1001-2036.2018.01.015
- VernacularTitle:葛根素对臂丛撕脱后脊髓运动神经元GAP-43及NGF蛋白表达的影响
- Author:
Longju CHEN
1
,
2
;
Chuanqi CHEN
;
Yunxia TAN
;
Taiding WU
;
Zunji KE
Author Information
1. 201203上海,上海中医药大学基础医学院生化教研室
2. 湖北民族学院医学院人体解剖学教研室
- Keywords:
Puerarin;
Brachial plexus injury;
Motor neuron;
Growth associated protein-43;
Nerve growth factor
- From:
Chinese Journal of Microsurgery
2018;41(1):62-65
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of Puerarin(Pue)on survival rate,expression of GAP-43 and NGF in spinal motoneurons following brachial roots avulsion. Methods From March, 2014 to December, 2015, 192 adult Sprague Dawley rats were divided into four groups: Avulsion, Pue 50 treatment, Pue 100 and Pue 200 groups. The right C5-C7nerve roots were avulsed through the methods of cervical dorsal approach. Puerarin or normal saline was given immediatedly once daily to the rats respectively by the intraperitoneal injection. The rats were killed at 1, 2, 4 and 6 weeks after injury. The paraffin sections of C7 segment were stained with neutral red. Expression of GAP-43 and NGF were detected by Western blot. Results Compared to the avuled group, the motoneuron survival rates of Pue 100 and Pue 200 dose treatment groups increased on the second week and the sixth week(P < 0.05 or P < 0.01), and the Pue 200 dose treatment group increased on the fourth week(P < 0.01).In the anterior horn of all three groups, expression of GAP-43 increased from the 1st week to the 6 week after the operation, and reached the peak at the 4th week, and decreased at the 6th week. Compared to the avuled group, expression of GAP-43 protein increased in the Pue 100 and Pue 200 dose treatment groups at the 1st week and 2nd week,the expression of NGF protein increased in the three Pue treatment groups at four time points(P < 0.05 or P < 0.01). Conclusion Puerarin can improve the survival rates of spinal motoneurons after the brachial plexus root avulsion, and the expression of GAP-43 and NGF increased, suggesting that Puerarin may play an role in the repair of brachial plexus avulsions by promoting the ex-pression of nerve growth factors.
