Expression of scavenger receptor class type B1 in esophageal squamous cell carcinoma and its effects on cell proliferation and invasion
10.3760/cma.j.issn.0254-1432.2018.08.007
- VernacularTitle:B类 Ⅰ 型清道夫受体在食管鳞状细胞癌中的表达与其对细胞增殖和侵袭的影响
- Author:
Yu TANG
1
,
2
;
Zhen LI
;
Zuxuan SHI
Author Information
1. 450003 郑州 ,河南省人民医院内分泌科
2. 阜外华中心血管病医院内分泌科
- Keywords:
Cell proliferation;
Cell cycle;
Scavenger receptor class type B 1;
Esophageal squamous cell carcinoma;
Cell invasion
- From:
Chinese Journal of Digestion
2018;38(8):535-542
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of scavenger receptor class type B 1 (SR-B1) in esophageal squamous cell carcinoma (ESCC) ,and its effects on proliferation and invasion of ESCC cells . Methods From May 2012 to August 2017 ,63 ESCC patients who underwent surgical resection in Henan Provincial People′s Hospital were enrolled and ESCC tissues and corresponding normal tissues were collected . The expression of SR-B1 protein in collected tissues and ESCC cells were detected by immunohistochemistry and Western blotting .ESCC EC1 cells and TE1 cells were transfected with SR-B1 small interfering RNA (siRNA) ,control siRNA ,pcDNA3 .1 and pcDNA3 .1-SR-B1 ,respectively .After transfection ,the expression of SR-B1 protein was examined by Western blotting .The cell proliferation , cell cycle and invasion ability of ESCC EC1 cells and TE1 cells after transfection were determined by cell counting kit-8 (CCK-8) assay ,flow cytometry and Transwell chamber .Chi-square test and t test were performed for statistical analysis .Results The positive rate of SR-B1 protein expression in ESCC tissues was 60 .3％ (38/63) ,which was higher than that of corresponding normal tissues (30 .2％ ,19/63) ,and the difference was statistically significant (χ2=11 .565 , P=0 .001) .The expression of SR-B1 protein in ESCC cells Eca109 ,EC9706 ,EC1 ,TE1 and KYSE70 were 0 .244 ± 0 .012 ,0 .285 ± 0 .018 ,0 .455 ± 0 .016 ,0 .479 ± 0 .019 and 0 .390 ± 0 .022 ,respectively ,which were all higher than that of normal esophageal epithelial cell Het-1A (0 .027 ± 0 .011) ,and the differences were statistically significant (t=23 .252 ,21 .633 ,39 .081 ,36 .010 and 25 .591 ;all P<0 .01) .The expression of SR-B1 protein in ESCC EC1 and TE1 was downregulated by SR-B1 siRNA . The downregulation of SR-B1 protein expression suppressed the proliferation of ESCC EC1 cells and TE1 cells ,increased the percentage of cells at G0/G1 phase of ESCC EC1 cells and TE1 cells ((64 .92 ± 1 .68)％ and (64 .34 ± 0 .94)％ ) ,and reduced the invasion abilities of EC1 and TE1 cells (33 .33 ± 7 .51 and 21 .67 ± 4 .04) .The upregulation of SR-B1 expression promoted the proliferation of ESCC EC1 cells and TE1 cells ,reduced the percentage of cells at G0/G1 phase ((31 .72 ± 1 .30)％ and (33 .12 ± 1 .04)％ ) ,and enhanced cell invasion abilities (285 .33 ± 28 .10 and 247 .33 ± 28 .29) .Conclusions The overexpression of SR-B1 in ESCC may be associated with the occurrence and development of ESCC .Treatment targeting SR-B1 may be a novel therapeutic strategy in ESCC .
