Imaging of needle-based confocal laser endomicroscopy in rabbit models
10.3760/cma.j.issn.1007-5232.2018.04.009
- VernacularTitle:细针型共聚焦激光显微内镜在体成像的动物实验研究
- Author:
Mingming ZHANG
1
;
Ning ZHONG
;
Xiuli ZUO
;
Xiao WANG
;
Rui JI
;
Yanbo YU
;
Zhen ZHANG
;
Yanqing LI
Author Information
1. 山东大学齐鲁医院消化科
- Keywords:
Animal experimentation;
Needle-based confocal laser endomicroscopy;
In vivo imaging
- From:
Chinese Journal of Digestive Endoscopy
2018;35(4):266-269
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the feasibility of needle-based confocal laser endomicroscopy (nCLE) for imaging of intra-abdominal tissues and organs in rabbit models in vivo. Methods The nCLE miniprobe was inserted through the 19-gauge needle into various intra-abdominal tissues and organs[omentum majus, liver, pancreas and psoas major (skeletal muscle)]. The nCLE images were acquired and real-time sequences of respective locations were recorded. Finally, nCLE image characteristics were compared with histopathologic findings. Results nCLE was successfully performed in intra-abdominal tissues and organs of five rabbit models. The microscopic structures of cells, glands and microvessels in the omentum majus, liver, pancreas and psoas major ( skeletal muscle) were visualized clearly, respectively. Characteristics of various intra-abdominal tissues and organs were displayed on nCLE images, which were correlated well with histological findings. Conclusion Imaging of intra-abdominal tissues and organs with nCLE in vivo is feasible in future clinical practice.
