Application value of haptoglobin as bile and serum biomarkers for biliary tract carcinoma
10.3760/cma.j.issn.1007-5232.2018.03.005
- VernacularTitle:触珠蛋白作为胆道肿瘤胆汁和血清生物标志物的研究
- Author:
Xiao ZHENG
1
;
Yechen WU
;
Daojian GAO
;
Bing HU
Author Information
1. 第二军医大学附属东方肝胆外科医院内镜科
- Keywords:
Biliary tract neoplasms;
Haptoglobin;
Biomarker;
Proteomics;
Tandem mass spectrometry;
liquid chromatography
- From:
Chinese Journal of Digestive Endoscopy
2018;35(3):171-174
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect cancer-related biomarkers for early diagnosis of biliary tract carcinoma. Methods Bile and serums were obtained from patients with histopathologically proven malignant or benign biliary diseases, and clinical data of patients were recorded. Two-dimensional liquid chromatography tandem mass spectrometry(2D-LC-MS/MS)was applied to detect the differential protein profiles in the bile. Western blot was used to validate the results in the biles and serums. Results Ten differential protein profiles were detected in 4 protein groups. Among them, the expression of haptoglobin significantly increased in the bile of patients with malignancy. This result was further verified using Western blot. Conclusion Haptoglobin may be a potential biomarker for biliary tract carcinoma.
