The diagnostic value of automated quantitative DNA cytometry for pancreatic malignancy
10.3760/cma.j.issn.1007-5232.2018.03.002
- VernacularTitle:全自动DNA定量技术对胰腺恶性肿瘤的诊断价值
- Author:
Xiaoyin ZHANG
1
;
Min ZHAO
;
Xin FU
;
Li YANG
;
Qiao YANG
;
Na LIU
;
Changcun GUO
;
Xiaoru KE
;
Xin WANG
;
Xuegang GUO
;
Kaichun WU
;
Daiming FAN
;
Hongbo ZHANG
Author Information
1. 空军军医大学西京医院消化内科
- Keywords:
Pancreatic neoplasms;
Pathology;
DNA probes;
Cytodiagnosis
- From:
Chinese Journal of Digestive Endoscopy
2018;35(3):157-162
- CountryChina
- Language:Chinese
-
Abstract:
Objective To estimate the diagnostic value of cytology, DNA-ICM(DNA-image cytometry),cytology combined with DNA-ICM for pancreatic malignancy,and to explore the cut-off value for DNA-ICM. Methods Patients with suspicious pancreatic malignancy were retrospectively identified. In total,145 EUS-FNA specimens acquired from 140 separate patients were examined by cytology and DNA-ICM. Diagnostic values among cytology, DNA-ICM and the combination of the techniques in detecting pancreatic malignancy were compared. Results Compared with cytology, DNA-ICM had a lower sensitivity (63.0% VS 82.4%)and accuracy(69.7% VS 85.5%). After combining the techniques, the diagnostic value for pancreatic malignancy significantly improved compared with that by cytology(0.941 VS 0.912, P=0.007 0)or DNA-ICM only(0.941 VS 0.815, P<0.000 1). By using the Youden index, the cut-off value for DNA-ICM to detect pancreatic malignancy was one cell with DI(DNA index)≥2.5. Notably,with this standard, the sensitivity and accuracy of DNA-ICM significantly increased to 72.3% and 77.2%, and those of the combined techniques increased to 91.6% and 93.1%, respectively. Conclusion Automated DNA-ICM is an objective and effective method for pancreatic malignancy. Although DNA-ICM has a lower diagnostic value than that of conventional cytology, an improved value was obtained after combining the techniques.
