Relationships between differences in tumorigenicity and immunogenetic backgrounds of nude mice

VernacularTitle:免疫遗传背景差异对裸鼠成瘤性影响的研究
Author: Jie WEI ¹ ; Hong WANG ; Jinping FAN ; Tao FAN ; Chunnan LIANG ; Zuomin LIU ; Bingfei YUE
Author Information

1. 中国食品药品检定研究院实验动物质量检测室
Keywords: Nude mice; Tumorigenicity; Immune; Genetics
From: Chinese Journal of Microbiology and Immunology 2018;38(11):835-840
CountryChina
Language:Chinese
Abstract: Objective To study the relationships between differences in tumorigenicity and immu-nogenetic backgrounds of nude mice. Methods According to the Chinese Pharmacopoeia, positive and neg-ative groups were set up in both Laboratory A and B with ten nude mice in each group. Organ tissues were collected for clinicopathological analysis. Blood samples were collected and detected using flow cytometry. DNA was extracted and analyzed with 23 STR markers. Results The positive group of Laboratory B was in-valid (7/10 tumor formation). The two laboratories showed no significant difference in the results of patho-logical analysis, but had significant differences in CD25, CD8, CD4, Th1 and Th2. There were 13 and 18 polymorphic sites respectively found in nude mice of Laboratory A and B. Further analysis of the non-tumor-bearing nude mice in Laboratory B positive group revealed that CD25, Th2, D3Mit29 and D5Mit48 were the specific indexes. Conclusion Differences in tumorigenicity might be related to the diversity of immunoge-netic backgrounds of nude mice.