Influences of IL-17 on regulatory T cell proliferation and IL-10 secretion during the very early stage of Chlamydia trachomatis respiratory tract infection
10.3760/cma.j.issn.0254-5101.2018.11.002
- VernacularTitle:衣原体呼吸道感染早期IL-17对调节性T细胞及其IL-10分泌的影响
- Author:
Hong ZHANG
1
;
Gaoju PANG
;
Xiaoyu ZHA
;
Wen-Hao NIU
;
Tengli LIU
;
Huili ZHAO
;
Hong BAI
Author Information
1. 300070 天津医科大学基础医学院免疫学系,国家教育部免疫微环境与疾病重点实验室,天津市细胞和分子免疫学重点实验室
- Keywords:
Chlamydia;
Treg;
IL-17;
Th17
- From:
Chinese Journal of Microbiology and Immunology
2018;38(11):807-811
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influences of IL-17 on regulatory T ( Treg) cells during Chlamydia trachomatis infection. Methods Wild-type ( WT) C57BL/6 mice and IL-17-/- mice were in-tranasally injected with 1×103inclusion forming units (IFU) of Chlamydia muridarum (Cm) to establish the mouse model of Chlamydia trachomatis respiratory tract infection. Mouse spleen and lung single cells were prepared. The percentages of CD4+CD25+T and CD4+CD25+Foxp3+T cells were detected by flow cytome-try. Expression of Foxp3 and TGF-β at mRNA level in lung was detected by RT-PCR. The levels of IL-10 in bronchoalveolar lavage fluid samples were detected by ELISA. Results Compared with the WT mice, the IL-17-/- mice had higher percentages of CD4+CD25+T and CD4+CD25+Foxp3+T cells in spleen and lung on the third day of Cm infection. Both of the expression of Foxp3 at mRNA level in lung and the secretion of IL-10 in bronchoalveolar lavage fluid were increased in IL-17-/- mice as compared with those in WT mice. No significant difference in the expression of TGF-β at mRNA level in lung tissues was found between the two groups. Conclusion IL-17 might inhibit the proliferation of Treg cells and the secretion of IL-10 in the very early stage of Cm respiratory tract infection.
