Detection of peripheral CD14+CD277+monocyte-macrophage ratio with γδ TCR tetramer and analy-sis of its relationship with treatment outcome
10.3760/cma.j.issn.0254-5101.2018.11.001
- VernacularTitle:利用γδ TCR四聚体检测分析外周血中CD14+CD277+单核-巨噬细胞的比例及其与治疗转归的关系
- Author:
Ling MAO
1
;
Zhixiong MEI
;
Xiaoxin TU
;
Yimin FANG
;
Yichuan GAN
;
Yanming SHEN
;
Xiaomin LAI
Author Information
1. 510080 广州,中山大学中山医学院微生物学教研室,热带病防治研究教育部重点实验室,结核病研究所,海洋微生物功能分子广东省高校重点实验室,广东省重大传染病预防和控制技术中心
- Keywords:
Tuberculosis;
γδ TCR tetramer;
Flow cytometry;
Monocyte-macrophage;
Treatment outcome
- From:
Chinese Journal of Microbiology and Immunology
2018;38(11):801-806
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of peripheral CD14+monocyte-macrophages in the recognition of phosphorylated antigen by γδ T cells and its relationship with treatment outcome. Methods Three kinds of γδ TCR tetramers were used to stain PBMC collected from patients with tuberculosis ( TB) and neonatal umbilical cord blood samples. The proportions of various TB-specific antigen presenting cells (APC) in peripheral blood were analyzed, and their relationships with treatment outcome were assessed based upon clinical data. Results CD14+monocyte-macrophages both in tuberculosis patients′ peripheral blood and neonatal umbilical cord blood were the strongest binding cells to CD277 antibody and γδ TCR tet-ramers. The median (P50) of CD14+monocyte-macrophages reached the highest peak after taking anti-tu-berculosis treatment for about one month and patients′condition was improved obviously during this period. Conclusion This study elucidated that CD14+monocyte-macrophages accounted for the largest proportion of APC when γδ T cells recognized phosphorylated antigens, which provided reference data for further study on the mechanism of γδ T cells restrictively recognizing phosphorylated antigen and their significance in innate and adaptive immunity.
