Glycyrrhiza uralensis Fisch L. crude polysaccharides enhance mouse immunity and immune responses induced by HPV-DNA vaccine
10.3760/cma.j.issn.0254-5101.2018.10.010
- VernacularTitle:乌拉尔甘草粗多糖提高小鼠免疫力并增强HPV-DNA疫苗免疫应答
- Author:
Shanshan CAI
1
;
Adila·Aipire
;
Alimu·Aimaier
;
Qiuyan CHEN
;
Yijie LI
;
Jinyao LI
Author Information
1. 830046 乌鲁木齐,新疆大学生命科学与技术学院,新疆生物资源基因工程重点实验室
- Keywords:
Glycyrrhiza uralensis Fisch L.;
Crude polysaccharides;
Immunity;
DNA vaccine;
Im-mune response
- From:
Chinese Journal of Microbiology and Immunology
2018;38(10):774-781
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Glycyrrhiza uralensis Fisch L. crude polysac-charides (GUCP) as an adjuvant on the immunity of mice and the immune responses induced by human pap-illoma virus ( HPV)-DNA vaccine. Methods ICR mice were injected with different concentrations of GUCP by different ways to detect the influences of GUCP on body weight, organ indexes and the numbers of immune cells in spleen. C57BL/ 6 mice were co-immunized with HPV-DNA vaccine and GUCP to detect the adjuvant efficacy on antigen-specific cellular and humoral immune responses. Results GUCP in all injected groups had no side effect on mouse body weight and liver, heart, lung and kidney indexes, but intraperitone-al injection of GUCP significantly increased spleen and thymus indexes and the numbers of B cells, CD4+ T cells, macrophages and dendritic cells in spleen. Subcutaneous injection of GUCP significantly increased the numbers of B cells and macrophages in spleen and intragastric administration significantly increased the num-bers of CD4+ and CD8+ T cells in spleen. Furthermore, GUCP as an adjuvant enhanced the antigen-specific CD4+ and CD8+ T cell responses and the levels of IgG, IgG1 and IgG2a induced by HPV-DNA vaccine at a certain degree. Conclusion GUCP enhanced the immunity of mice and the antigen-specific cellular and hu-moral immune responses induced by HPV-DNA vaccine. These results suggested that GUCP might be used as an adjuvant for DNA vaccine.
